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公开(公告)号:US20250019729A1
公开(公告)日:2025-01-16
申请号:US18658087
申请日:2024-05-08
Inventor: Leticia R. CARRILLO-MAX
IPC: C12P7/625 , C07K14/195 , C07K14/245 , C12N15/70
Abstract: An embodiment of the present disclosure provides an isolated nucleic acid molecule comprising a sequence encoding a protein having a PhaP1 domain and a HlyA domain, wherein the HlyA domain differs from the wild-type HlyA domain of E. coli (SEQ ID NO.: 1) SEQ ID NO. 1: LAYGSQGDLNPLINEISKIISAAGSFDVKEERTAAS LLQLSGNASDFSYGRNSITLTTSA in at least the wild-type amino acid residue S (serine) at position 25 (underlined above) is replaced with the amino acid residue N (asparagine).