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公开(公告)号：US12060596B2
公开(公告)日：2024-08-13
申请号：US16399145
申请日：2019-04-30
发明人： Alexander Brett Foster , Cristina Serrano Amatriain , Gary J. Smith , Paul Sheldon Pearlman , Mark Paul Taylor , Jonathan Combe , Daniel Bawdon
CPC分类号： C12P7/625 , C12N1/20 , C12N9/0006 , C12N9/1029 , C12N9/13 , C12N9/88 , C12P7/04 , C12P7/065 , C12P7/42 , C12Y101/01001 , C12Y203/01016 , C12Y208/03015 , C12Y401/01004 , C12N2500/02 , C12N2500/05 , C12N2500/12 , C12N2500/24
摘要： Provided herein are methods for increasing the yield of an extracellular product synthesized by an organism cultured in a continuous aerobic fermentation system. The extracellular product yield is increased through the use of an organism modified to decreased production of polyhydroxyalkanoate, to increase production of the extracellular product, and to include promoters that can be inducible in response to nutrient limitation conditions. The extracellular product yield is also increased by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are non-naturally occurring organisms that have been modified for use with the provided methods, and extracellular products made using the provided methods.
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2.发明授权公开(公告)号：US12031123B2
公开(公告)日：2024-07-09
申请号：US18319444
申请日：2023-05-17
IPC分类号： C12P7/625 , C07K14/21 , C12N1/20 , C12N1/21 , C12N9/04 , C12N9/10 , C12N9/90 , C12N15/52 , C12P7/52 , C12R1/19
CPC分类号： C12N1/20 , C07K14/21 , C12N1/205 , C12N9/0006 , C12N9/1029 , C12N9/13 , C12N9/90 , C12N15/52 , C12P7/52 , C12P7/625 , C12Y101/01036 , C12Y203/01009 , C12Y203/01016 , C12Y208/03001 , C12Y504/99002 , C12R2001/19
摘要： The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.
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公开(公告)号：US11913056B2
公开(公告)日：2024-02-27
申请号：US18031545
申请日：2022-06-28
发明人： Jin Yin , Yu Wang , Xiaocui Xie , Huayu Zhang , Yakun Wu , Tian Liu , Pingan Tang
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N9/88 , C12N15/74 , C12Y101/01036 , C12Y402/01017
摘要： Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.
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公开(公告)号：US11732280B2
公开(公告)日：2023-08-22
申请号：US17457608
申请日：2021-12-03
发明人： Markus D. Herrema
CPC分类号： C12P7/625
摘要： Embodiments of the invention relate generally to methods to generate microorganisms and/or microorganism cultures that exhibit the ability to produce polyhydroxyalkanoates (PHA) from carbon sources at high efficiencies. In several embodiments, preferential expression of, or preferential growth of microorganisms utilizing certain metabolic pathways, enables the high efficiency PHA production from carbon-containing gases or materials. Several embodiments relate to the microorganism cultures, and/or microorganisms isolated therefrom.
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公开(公告)号：US20230220154A1
公开(公告)日：2023-07-13
申请号：US18153588
申请日：2023-01-12
申请人： Meredian, Inc.
摘要： A cake material is disclosed, made up of polyhydroxyalkanoate (PHA) cake that is formed directly from biomass and subsequent purification processes absent any heated drying step, with a moisture content of no less than about 5% by weight, and a Dv (90) particle size of no more than about 8 microns.
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公开(公告)号：US11697831B2
公开(公告)日：2023-07-11
申请号：US17265135
申请日：2019-07-31
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12Y101/01027 , C12Y208/03001
摘要： Disclosed is the production by fermentation of poly D-lactic acid (PDLA) and poly L-lactic acid (PLLA). In particular, there is provided engineered (prokaryotic or eukaryotic) cells for the direct synthesis of PLLA polymers and engineered eukaryotic cells for the direct synthesis of PDLA polymers starting from a carbon source, including residual biomasses of the different production chains.
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公开(公告)号：US20230167046A1
公开(公告)日：2023-06-01
申请号：US17378958
申请日：2019-01-23
申请人： IOI Oleo GmbH
发明人： Dirk LOCHMANN , Sebastian REYER , Michael STEHR
摘要： The invention relates to a method for producing polyol esters, especially polyglycerol esters, of 3-hydroxybutyric acid as well as the products thus obtained and their use.
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公开(公告)号：US20230151395A1
公开(公告)日：2023-05-18
申请号：US17379083
申请日：2019-01-23
申请人： IOI Oleo GmbH
发明人： Dirk LOCHMANN , Sebastian REYER , Michael STEHR
摘要： The invention relates to a method for producing glycerides of 3-hydroxybutyric acid as well as the products thus obtained and their use.
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公开(公告)号：US20230061702A1
公开(公告)日：2023-03-02
申请号：US17891435
申请日：2022-08-19
申请人： Meredian, Inc.
摘要： A method for recovering polyhydroxyalkanoates from a biomass is disclosed. According to the method, polynucleotide chains are cleaved by addition of an endonuclease. A lysing agent is used to disrupt cell walls of the microorganism cells and release the intracellular polyhydroxyalkanoates from the cells. Proteins are also degraded by addition of a peptidase. The polyhydroxyalkanoates are then separated from cellular debris of the cells. According to the present disclosure, this method is carried out without the use of organic solvents in the cleaving, lysing, and degrading steps.
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公开(公告)号：US20220315876A1
公开(公告)日：2022-10-06
申请号：US17656462
申请日：2022-03-25
申请人： LanzaTech, Inc.
摘要： The disclosure provides for methods and a system for storing energy in the form of a biopolymer. The method comprises intermittently processing electric energy generated from a renewable and/or non-renewable energy source in an electrolysis process to produce at least H2, Oor CO; intermittently passing H2, O2, or CO from the electrolysis process to a bioreactor containing a bacterial culture capable of producing a biopolymer; and fermenting the culture. The disclosure further provides a system for storing energy in the form of biopolymer comprising an electrolysis process in intermittent fluid communication with a renewable and/or non-renewable energy source for producing at least one of H2, O2, or CO; a bioreactor, in intermittent fluid communication with the electrolysis process and/or in continuous fluid communication with an industrial plant, comprising a reaction vessel suitable for intermittently growing, fermenting, and/or culturing and housing a microorganism capable of producing a biopolymer.
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