公开(公告)号：US20180298069A1

公开(公告)日：2018-10-18

申请号：US15580110

申请日：2016-06-09

Applicant: FIRMENICH SA

Inventor： Hyo-Young JEONG ,  Patrick PFISTER ,  Matthew ROGERS

IPC: C07K14/47 ,  C07K14/705 ,  C12N15/86 ,  C12N15/11 ,  C12N9/22 ,  G01N33/50

Abstract: Provided herein is a cell line with improved odorant receptor function comprising an activated endogenous RTP1 gene, which further expresses an RTP1 protein. Further provided herein is a method for specifically activating an endogenous RTP1 gene in a eukaryotic cell using a CRISPR/Cas9 derived technique. Also provided herein is a method for identifying compounds with desired effects such as perfume or aroma modulators in said cell line.