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公开(公告)号：US20240309425A1
公开(公告)日：2024-09-19
申请号：US18370325
申请日：2023-09-19
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/6806 , B01L3/00 , B01L7/00 , C12Q1/686
CPC classification number: C12Q1/6806 , B01L3/502738 , C12Q1/686 , B01L3/50273 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US20170349934A1
公开(公告)日：2017-12-07
申请号：US15590998
申请日：2017-05-09
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
CPC classification number: C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US20210324446A1
公开(公告)日：2021-10-21
申请号：US17166992
申请日：2021-02-03
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/6806 , C12Q1/686 , B01L3/00
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US10344318B2
公开(公告)日：2019-07-09
申请号：US15590998
申请日：2017-05-09
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/686 , C12Q1/6806 , B01L3/00 , B01L7/00
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US09677119B2
公开(公告)日：2017-06-13
申请号：US14180262
申请日：2014-02-13
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
CPC classification number: C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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Patent Agency Ranking
公开(公告)号：US11795494B2
公开(公告)日：2023-10-24
申请号：US17166992
申请日：2021-02-03
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/6806 , C12Q1/686 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/6806 , B01L3/502738 , C12Q1/686 , B01L3/50273 , B01L7/52 , B01L2300/087 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629 , C12Q1/6806 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US09644231B2
公开(公告)日：2017-05-09
申请号：US14713887
申请日：2015-05-15
Applicant: Fluidigm Corporation
Inventor： Kenneth J. Livak , Stacey N. Meyers , Xiaohui Wang , Jun Wang
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and using a DNA polymerase with high strand displacement activity and low 5′-nuclease activity, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
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公开(公告)号：US09587272B2
公开(公告)日：2017-03-07
申请号：US14340446
申请日：2014-07-24
Applicant: Fluidigm Corporation
Inventor： Kenneth J. Livak , Stacey N. Meyers , Jun Wang , Xiaohui Wang
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了包含调节序列和核苷酸标签识别序列的具有解链温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。
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公开(公告)号：US20150147755A1
公开(公告)日：2015-05-28
申请号：US14340446
申请日：2014-07-24
Applicant: Fluidigm Corporation
Inventor： Kenneth J. Livak , Stacey N. Myers , Jun Wang , Xiaohui Wang
IPC: C12Q1/68
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。
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公开(公告)号：US20150361486A1
公开(公告)日：2015-12-17
申请号：US14713887
申请日：2015-05-15
Applicant: FLUIDIGM CORPORATION
Inventor： Kenneth J. Livak , Stacey N. Meyers , Xiaohui Wang , Jun Wang
IPC: C12Q1/68
CPC classification number: C12Q1/6818 , C12Q2525/151
Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and using a DNA polymerase with high strand displacement activity and low 5′-nuclease activity, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列互补的序列区段和当序列片段不与探针核苷酸杂交时的尾段 并使用探针寡核苷酸作为引物，并使用具有高链置换活性和低5'-核酸酶活性的DNA聚合酶，在PCR扩增反应中扩增标记的靶核酸序列，并检测扩增产物 ; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。
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