公开(公告)号：US11875298B2

公开(公告)日：2024-01-16

申请号：US16518544

申请日：2019-07-22

申请人： FORSCHUNGSZENTRUM JULICH GMBH

发明人： Solvej Siedler ,  Georg Schendzielorz ,  Stephan Binder ,  Lothar Eggeling ,  Stephanie Bringer-Meyer ,  Michael Bott

IPC分类号： C12Q1/6897 ,  G06Q10/0639 ,  G06N5/045 ,  A61K31/07 ,  C12N15/70 ,  G06N3/006 ,  G06Q10/04 ,  C12Q1/26 ,  C12Q1/68

CPC分类号： G06Q10/06395 ,  A61K31/07 ,  C12N15/70 ,  C12Q1/6897 ,  C12Y101/00 ,  G06N3/006 ,  G06N5/045 ,  G06Q10/04 ,  C12Q1/26 ,  C12Q1/68

摘要： An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

公开(公告)号：US10385349B2

公开(公告)日：2019-08-20

申请号：US14424559

申请日：2013-08-16

申请人： FORSCHUNGSZENTRUM JULICH GMBH

发明人： Solvej Siedler ,  Georg Schendzielorz ,  Stephan Binder ,  Lothar Eggeling ,  Stephanie Bringer-Meyer ,  Michael Bott

IPC分类号： C12N15/70 ,  C12Q1/6897

摘要： The present invention relates to an NADP(H) nanosensor comprising i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

公开(公告)号：US20150299715A1

公开(公告)日：2015-10-22

申请号：US14424559

申请日：2013-08-16

申请人： FORSCHUNGSZENTRUM JULICH GMBH

发明人： Solvej Siedler ,  Georg Schendzielorz ,  Stephan Binder ,  Lothar Eggeling ,  Stephanie Bringer-Meyer ,  Michael Bott

IPC分类号： C12N15/70 ,  C12Q1/68

CPC分类号： C12N15/70 ,  C12Q1/6897

摘要： The present invention relates to an NADP(H) nanosensor comprising i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

摘要翻译： 本发明涉及一种NADP（H）纳米传感器，其包含i）调节器能够结合的核酸序列，其中调节剂的氧化态取决于NADP（H）的可用性; ii）在RNA聚合酶能够结合的核酸序列i）之后的启动子序列，其中RNA聚合酶对启动子序列的亲和力受调节剂的氧化状态的影响; iii）在启动子序列ii）控制下的编码自身荧光蛋白的核酸序列。 本发明还涉及细胞，用于分离编码NADP（H） - 依赖性酶的基因的方法和NADP（H）纳米传感器的用途）。