公开(公告)号：US20210380524A1

公开(公告)日：2021-12-09

申请号：US17123098

申请日：2020-12-15

Applicant: Fudan University

Inventor： Fener CHEN ,  Dang CHENG ,  Minjie LIU ,  Meifen JIANG ,  Zedu HUANG ,  Zexu WANG ,  Jiaqi WANG

IPC: C07C227/04 ,  B01J19/00

Abstract: A method for preparing L-carnitine using a micro-reaction system. (R)-4-halo-3-hydroxybutyrate was subjected to quaternization and hydrolysis in an aqueous trimethylamine solution in the presence of an inorganic base in a micro-channel reactor to produce the L-carnitine.

公开(公告)号：US20180340196A1

公开(公告)日：2018-11-29

申请号：US15871039

申请日：2018-01-14

Applicant: FUDAN UNIVERSITY

Inventor： Fener CHEN ,  Zedu HUANG ,  Ge MENG ,  Minjie LIU ,  Zhining LI ,  Zexu WANG ,  Haihui PENG ,  Fangjun XIONG ,  Yan WU ,  Yuan TAO

IPC: C12P7/62 ,  C07C69/732 ,  C07C67/28 ,  C12N9/04

CPC classification number: C12P7/62 ,  C07C67/28 ,  C07C69/732 ,  C12N9/0006 ,  C12Y101/0108 ,  G01N30/7206 ,  G01N30/88 ,  G01N2030/025

Abstract: The present disclosure relates to the technical field of biochemical engineering and particularly discloses a preparation method for (R)-3-hydroxyl-5-hexenoate. In the method of the present disclosure, the (R)-3-hydroxyl-5-hexenoate is prepared by catalytic reduction of 3-carbonyl-5-hexenoate by ketoreductase with 3-carbonyl-5-hexenoate as the substrate. The amino acid sequence of ketoreductase is shown in SEQ ID NO.1. In the present disclosure, the (R)-3-hydroxyl-5-hexenoate having a very high chiral purity is obtained by asymmetric reduction by ketoreductase as the biocatalyst. The present disclosure has the advantages of easy operation, mild reaction conditions, high reaction yield and good practical industrial application value.