公开(公告)号：US20210017494A1

公开(公告)日：2021-01-21

申请号：US17063204

申请日：2020-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. VODYANYK ,  Xin ZHANG ,  Andrew J. BRANDL ,  Deepika RAJESH ,  Bradley SWANSON ,  Christie MUNN ,  Sarah A. BURTON ,  Wen Bo WANG

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789 ,  C12N15/85 ,  C12N5/0781 ,  C12N15/86

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US20200263139A1

公开(公告)日：2020-08-20

申请号：US16339676

申请日：2017-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. VODYANYK ,  Xin ZHANG ,  Andrew J. BRANDL ,  Deepika RAJESH ,  Bradley SWANSON ,  Christie MUNN ,  Sarah BURTON ,  Wen Bo WANG

IPC: C12N5/074 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/0789

Abstract: Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.