公开(公告)号：US20200263139A1

公开(公告)日：2020-08-20

申请号：US16339676

申请日：2017-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. VODYANYK ,  Xin ZHANG ,  Andrew J. BRANDL ,  Deepika RAJESH ,  Bradley SWANSON ,  Christie MUNN ,  Sarah BURTON ,  Wen Bo WANG

IPC: C12N5/074 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/0789

Abstract: Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US20230183644A1

公开(公告)日：2023-06-15

申请号：US17931440

申请日：2022-09-12

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Deepika RAJESH ,  Christie MUNN ,  Sarah BURTON ,  Madelyn Elise DONEGAN ,  Makiko OHSHIMA ,  Coby CARLSON ,  Megan LIVINGSTON ,  Kimihiko TOMOTOSHI ,  Ali FATHI ,  Jing LIU ,  Wayne POON ,  Simon HILCOVE ,  Kiranmayee BAKSHY

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N2506/45 ,  C12N2501/2334 ,  C12N2501/22 ,  C12N2501/15 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2513/00 ,  C12N2503/02

Abstract: The present disclosure provides multicellular culture models for the study of neuroinflammation, such as to identify novel targets, biomarkers, and therapeutic agents for the diagnosis, prognosis, and treatment of neurodegenerative diseases. Further provided herein are assays for studying neuroinflammation using the present cell culture models.

公开(公告)号：US20240003871A1

公开(公告)日：2024-01-04

申请号：US18344703

申请日：2023-06-29

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM Holdings America Corporation

Inventor： Deepika RAJESH ,  Christie MUNN ,  Sarah BURTON ,  Ali FATHI ,  Rachel LEWIS ,  Robert BRADLEY ,  Jing LIU

IPC: G01N33/50 ,  C12N5/079

CPC classification number: G01N33/5058 ,  C12N5/0622 ,  C12N2506/45 ,  C12N2501/235 ,  C12N2500/90 ,  C12N2501/727 ,  C12N2533/90 ,  C12N2513/00 ,  C12N2501/13 ,  C12N2501/11 ,  C12N2501/25 ,  C12N2501/2301

Abstract: The present disclosure provides method for producing neural precursor cells with a glial bias from induced pluripotent cells and further differentiating the neural precursor cells to astrocytes. Further provided herein are methods for the use of the astrocytes for screening assays, models mimicking the human brain, and cell therapy.

公开(公告)号：US20220220440A1

公开(公告)日：2022-07-14

申请号：US17610123

申请日：2020-05-11

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM Holdings America Corporation

Inventor： Igor GUIREVICH ,  Sarah BURTON ,  Christie MUNN ,  Madelyn GOEDLAND ,  Katherine CZYSZ ,  Deepika RAJESH ,  Makiko OHSHIMA

IPC: C12N5/071 ,  G01N33/50 ,  A61K35/407 ,  A61P1/00

Abstract: The present disclosure provides methods of producing hepatocytes from induced pluripotent stem cells. Further provided herein are methods of using the hepatocytes for the treatment of a liver disease.

公开(公告)号：US20240191186A1

公开(公告)日：2024-06-13

申请号：US17760446

申请日：2022-05-05

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM HOLDINGS AMERICA CORPORATION

Inventor： Deepika RAJESH ,  Kiranmayee BAKSHY ,  Christie SAVIC ,  Sarah BURTON ,  Wayne POON ,  Simon HILCOVE

IPC: C12N5/079 ,  G01N33/50 ,  G01N33/68

CPC classification number: C12N5/0622 ,  G01N33/5058 ,  G01N33/6863 ,  C12N2506/45 ,  G01N2333/535 ,  G01N2333/5406 ,  G01N2333/5437 ,  G01N2333/57 ,  G01N2400/50

Abstract: The present disclosure provides iPSC-derived microglia comprising a protective CD33 allele. Further provided herein are assays for screening analytes and genes associated with the protective CD33 allele.

公开(公告)号：US20220389436A1

公开(公告)日：2022-12-08

申请号：US17826112

申请日：2022-05-26

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Sarah DICKERSON ,  Sarah BURTON ,  Christie MUNN ,  Madelyn GOEDLAND ,  Michael MCLACHLAN ,  Deepika RAJESH ,  Tom BURKE

IPC: C12N15/79

Abstract: Provided herein are methods of producing cell lines with stable expression of a transgene by removal of CpG motifs. In further methods, there are provided methods for cell lines with stable expression of a transgene by driving expression by novel promoters or by tagging endogenous genes.

公开(公告)号：US20220251516A1

公开(公告)日：2022-08-11

申请号：US17619096

申请日：2020-06-15

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM Holdings America Corporation

Inventor： Deepika RAJESH ,  Christie MUNN ,  Sarah BURTON ,  Madelyn GOEDLAND ,  Michael McLACHLAN ,  Abbey MUSINSKY ,  Kwi Hye KIM ,  Michael HANCOCK ,  Makiko OHSHIMA ,  Anne STROUSE ,  Sarah DICKERSON

IPC: C12N5/074 ,  C12N5/00

Abstract: The present disclosure provides methods of producing progenitor cells from induced pluripotent stem cells, wherein the progenitor cells comprise endothelial cells, pericytes, brain microvascular endothelial cells (BMECs), mesenchymal stem cells (MSCs), hematopoietic precursor cells (HPCs), microglia or neural precursor cells (NPCs).