公开(公告)号：US20240044773A1

公开(公告)日：2024-02-08

申请号：US18491962

申请日：2023-10-23

Applicant: FUJIFILM Corporation

Inventor： Shota KAMEI ,  Takashi MORIMOTO ,  Tomoyuki SHIMODA

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1434 ,  B01L3/502715 ,  G01N15/1429 ,  B01L2300/0654 ,  B01L2300/027 ,  G01N2015/0065

Abstract: Provided are a cell life-and-death determination method, a cell life-and-death determination device, and a cell life-and-death determination system. The cell life-and-death determination method includes: acquiring images of a cell captured in a plurality of focal planes including an in-focus plane of the cell in a direction opposite to a side on which the cell is irradiated with light; acquiring an image piece including a central portion and an outer peripheral portion of the cell from each of the images; connecting the image pieces in an order of an imaging direction of the focal plane to create a connected image for analysis; extracting a feature amount from the connected image for analysis; and determining whether the cell is alive or dead on the basis of the feature amount of the connected image for analysis and a predetermined range of the feature amount.