公开(公告)号：US20240052287A1

公开(公告)日：2024-02-15

申请号：US18494301

申请日：2023-10-25

Applicant: FUJIFILM Corporation

Inventor： Shota KAMEI ,  Takashi Morimoto ,  Tomoyuki Shimoda

IPC: C12M1/34 ,  C12M1/06 ,  C12M1/00 ,  C12M1/36

CPC classification number: C12M41/36 ,  C12M27/02 ,  C12M31/00 ,  C12M41/48

Abstract: Provided are a cell culture apparatus and a living cell concentration adjustment method. The cell culture apparatus includes: a first container that holds a cell suspension; a second container that holds a diluent diluting the cell suspension; a living cell concentration measurement device that individually determines whether a plurality of target cells in the cell suspension are alive or dead to measure a living cell concentration of the target cells in the cell suspension; a control device that holds and controls the living cell concentration and amount data of the cell suspension; a mixing unit that mixes the cell suspension and the diluent; and a culture container in which the diluted cell suspension is seeded to culture cells. The control device determines an amount of the cell suspension to be seeded and an amount of the diluent required to adjust the cell suspension to be seeded to a predetermined living cell concentration, on the basis of the living cell concentration and the amount data of the cell suspension, feeds the amount of the cell suspension and the amount of the diluent to the mixing unit, and seeds the diluted cell suspension.

公开(公告)号：US20240044773A1

公开(公告)日：2024-02-08

申请号：US18491962

申请日：2023-10-23

Applicant: FUJIFILM Corporation

Inventor： Shota KAMEI ,  Takashi MORIMOTO ,  Tomoyuki SHIMODA

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1434 ,  B01L3/502715 ,  G01N15/1429 ,  B01L2300/0654 ,  B01L2300/027 ,  G01N2015/0065

Abstract: Provided are a cell life-and-death determination method, a cell life-and-death determination device, and a cell life-and-death determination system. The cell life-and-death determination method includes: acquiring images of a cell captured in a plurality of focal planes including an in-focus plane of the cell in a direction opposite to a side on which the cell is irradiated with light; acquiring an image piece including a central portion and an outer peripheral portion of the cell from each of the images; connecting the image pieces in an order of an imaging direction of the focal plane to create a connected image for analysis; extracting a feature amount from the connected image for analysis; and determining whether the cell is alive or dead on the basis of the feature amount of the connected image for analysis and a predetermined range of the feature amount.