公开(公告)号：US20230126568A1

公开(公告)日：2023-04-27

申请号：US18146198

申请日：2022-12-23

Applicant: FUJIFILM Corporation

Inventor： Yuki IMAKURA ,  Shinji MIMA ,  Izumi OGURA ,  Nao YAMAZAKI

IPC: C12N5/071

Abstract: An object of the present invention is to provide a method of producing an intestinal epithelial cell, which has a large number of cells per area and a high accuracy of kinetic prediction for a CYP3A4 substrate drug such as midazolam, by inducing the differentiation of a pluripotent stem cell, as well as the intestinal epithelial cell, a cell sheet, an evaluation method for a test substance, a screening kit for a test substance, and a cell preparation. According to the present invention, there is provided a production method for an intestinal epithelial cell, including a first differentiation step of differentiating a pluripotent stem cell into an intestinal stem cell, a proliferation step of proliferating the intestinal stem cell obtained in the differentiation step, and a second differentiation step of differentiating the intestinal stem cell obtained in the proliferation step into an intestinal epithelial cell, in which the proliferation step is a step of bringing the intestinal stem cell into a specific state.

公开(公告)号：US20240302353A1

公开(公告)日：2024-09-12

申请号：US18666937

申请日：2024-05-17

Applicant: FUJIFILM Corporation

Inventor： Nao YAMAZAKI ,  Keiji SHIGESADA ,  Akira WAKABAYASHI ,  Shinji MIMA ,  Yuki IMAKURA

IPC: G01N33/50 ,  C12M1/00 ,  C12M1/04 ,  C12M1/12 ,  C12M3/06 ,  C12N5/071

CPC classification number: G01N33/5082 ,  C12M23/16 ,  C12M23/24 ,  C12M25/04 ,  C12M29/06 ,  C12N5/0679 ,  C12N2502/70

Abstract: An object of the present invention is to provide a cell culture device capable of reproducing, in vitro, a growth and proliferation environment of an anaerobic bacterium in a living body; a kit for cell culture; and a cell culture method. According to the present invention, there is provided a cell culture device including a plate having a concave portion for accommodating a culture medium, a cell accommodating container having a gas permeable membrane, a first member for holding the cell accommodating container, and a second member having a structure for regulating a gas concentration in an upper space of the cell accommodating container, in which, in a case where a cell sheet is formed on an upper surface of the gas permeable membrane, a space above the gas permeable membrane is in a sealed state in which gas does not pass through.

公开(公告)号：US20210147808A1

公开(公告)日：2021-05-20

申请号：US17158797

申请日：2021-01-26

Applicant: FUJIFILM Corporation

Inventor： Shinji MIMA ,  Yuki IMAKURA ,  Izumi OGURA

IPC: C12N5/071

Abstract: An object of the present invention is to provide a method for producing an intestinal epithelial cell in which the barrier function is maintained while the differentiation of a pluripotent stem cell into a liver cell is suppressed and an intestinal epithelial cell in which the barrier function is maintained while the differentiation into a liver cell is suppressed. According to the present invention, provided is the method for producing an intestinal epithelial cell, including a step 1 of differentiating a pluripotent stem cell into an intestinal stem cell and a step 2 of differentiating the intestinal stem cell obtained in the step 1 into an intestinal epithelial cell in a presence of one or more selected from the group consisting of a MEK1 inhibitor, a DNA methylation inhibitor, and a TGFβ receptor inhibitor, and EGF, in which during the step 2, a cell under differentiation is replated one or more times at the predetermined timing which is defined in the present specification.

公开(公告)号：US20240279613A1

公开(公告)日：2024-08-22

申请号：US18645071

申请日：2024-04-24

Applicant: FUJIFILM Corporation

Inventor： Nao YAMAZAKI ,  Shinji MIMA ,  Yuki IMAKURA

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2501/15 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2503/02 ,  C12N2506/23

Abstract: An object of the present invention is to provide a method of producing an enteroendocrine cell by using an intestinal stem cell induced from a pluripotent stem cell, a method of analyzing a disease mechanism, an evaluation method for a test substance, a screening method for a therapeutic drug for a digestive system disease, an enteroendocrine cell derived from a pluripotent stem cell, a culture medium or a culture medium kit, and a kit for producing an enteroendocrine cell. According to the present invention, there is provided a method of producing an enteroendocrine cell, the method including an intestinal stem cell or an intestinal epithelial cell, which is induced from a pluripotent stem cell, by using a culture medium that substantially does not contain a ligand of an epidermal growth factor but contains a cAMP activator and a DNA methyltransferase inhibitor.

公开(公告)号：US20220267704A1

公开(公告)日：2022-08-25

申请号：US17742123

申请日：2022-05-11

Applicant: FUJIFILM Corporation

Inventor： Izumi OGURA ,  Keiji SHIGESADA ,  Shinji MIMA ,  Yuki IMAKURA ,  Shun GOTO ,  Nao YAMAZAKI

IPC: C12M1/04 ,  C12M1/00 ,  C12M1/12

Abstract: An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation. According to the present invention, provided is a cell preservation or transportation instrument including a plurality of cell storage containers, a plate which has a plurality of recesses for holding the plurality of cell storage containers therein, and a flexible material sheet which seals upper opening portions of the plurality of cell storage containers and upper side wall portions formed by side walls of the recesses of the plate and allows ventilation between an inside and an outside of the plurality of cell storage containers.