公开(公告)号：US10234470B2

公开(公告)日：2019-03-19

申请号：US14412317

申请日：2012-07-04

申请人： Fangyang He ,  Yuping Wan ,  Caiwei Feng ,  Xiaoqin Luo ,  Jing Feng ,  Haifeng Cui ,  Nianqin Xu ,  Liangliang Zhu ,  Yumei Liu ,  Fangfang Jia ,  Zhengmiao Zhao

发明人： Fangyang He ,  Yuping Wan ,  Caiwei Feng ,  Xiaoqin Luo ,  Jing Feng ,  Haifeng Cui ,  Nianqin Xu ,  Liangliang Zhu ,  Yumei Liu ,  Fangfang Jia ,  Zhengmiao Zhao

IPC分类号： G01N33/94 ,  G01N33/577

摘要： The present invention discloses an enzyme-linked immunosorbent assay kit for detecting dinitolmide and use thereof. The enzyme-linked immunosorbent assay kit of the present invention comprises dinitolmide antibody and coating antigen and enzyme conjugate; wherein, the dinitolmide antibody is dinitolmide monoclonal antibody or dinitolmide polyclonal antibody; when the coating antigen is the conjugate of dinitolmide hapten and carrier protein, the enzyme conjugate is enzyme-labeled secondary antibody, or enzyme-labeled specific anti-dinitolmide monoclonal or polyclonal antibody; when the coating antigen is dinitolmide antibody or secondary antibody, the enzyme conjugate is enzyme-labeled dinitolmide hapten. The enzyme-linked immunosorbent assay kit has a simple structure, and it is convenient for use, cheap and portable. Its detection is high effective, accurate, and convenient. It can be used for on-site monitoring and is suitable for qualitative and quantitative screenings of a great number of samples. And thus, it will play an important role in the detection of dinitolmide.

公开(公告)号：US20150219678A1

公开(公告)日：2015-08-06

申请号：US14412317

申请日：2012-07-04

申请人： Fangyang He ,  Caiwei Feng ,  Yuping Wan ,  Nianqin Xu ,  Xiaoqin Luo ,  Jing Feng ,  Guangcan Tao ,  Houmei Yu ,  Yong Li ,  Sheng Fu ,  Peng Wu ,  Haifeng Cui

发明人： Fangyang He ,  Caiwei Feng ,  Yuping Wan ,  Nianqin Xu ,  Xiaoqin Luo ,  Jing Feng ,  Guangcan Tao ,  Houmei Yu ,  Yong Li ,  Sheng Fu ,  Peng Wu ,  Haifeng Cui

IPC分类号： G01N33/94 ,  G01N33/577

CPC分类号： G01N33/94 ,  G01N33/577 ,  G01N2430/00

摘要： The present invention discloses an enzyme-linked immunosorbent assay kit for detecting dinitolmide and use thereof. The enzyme-linked immunosorbent assay kit of the present invention comprises dinitolmide antibody and coating antigen and enzyme conjugate; wherein, the dinitolmide antibody is dinitolmide monoclonal antibody or dinitolmide polyclonal antibody; when the coating antigen is the conjugate of dinitolmide hapten and carrier protein, the enzyme conjugate is enzyme-labeled secondary antibody, or enzyme-labeled specific anti-dinitolmide monoclonal or polyclonal antibody; when the coating antigen is dinitolmide antibody or secondary antibody, the enzyme conjugate is enzyme-labeled dinitolmide hapten. The enzyme-linked immunosorbent assay kit has a simple structure, and it is convenient for use, cheap and portable. Its detection is high effective, accurate, and convenient. It can be used for on-site monitoring and is suitable for qualitative and quantitative screenings of a great number of samples. And thus, it will play an important role in the detection of dinitolmide.

摘要翻译： 本发明公开了一种用于检测二胺类化合物的酶联免疫吸附测定试剂盒及其应用。 本发明的酶联免疫吸附测定试剂盒包含二胺体抗体和包被抗原和酶结合物; 其中，二胺类抗体为二氨基甲酸单克隆抗体或二糖醇多克隆抗体; 当包被抗原是二氨基半胱氨酸半抗原和载体蛋白的结合物时，酶缀合物是酶标记的二抗或酶标记的特异性抗二糖醇单克隆或多克隆抗体; 当包被抗原是二胺抗体或二抗时，酶缀合物是酶标记的二胺体半抗原。 酶联免疫吸附测定试剂盒结构简单，使用方便，便宜便携。 其检测效率高，准确，方便。 它可用于现场监测，适用于大量样品的定性和定量筛选。 因此，它将在二硝基苯胺的检测中发挥重要作用。