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    Use of a Chimeric Gene 4-CL Encoding 4-Coumarate:CoA Ligase in Brassicaceae
    3.
    发明申请
    Use of a Chimeric Gene 4-CL Encoding 4-Coumarate:CoA Ligase in Brassicaceae 审中-公开
    使用编码4-香豆素的嵌合基因4-CL：芸苔科中的CoA连接酶

    公开(公告)号：US20110072538A1

    公开(公告)日：2011-03-24

    申请号：US12993802

    申请日：2008-11-25

    申请人： Hanzhong Wang ,  Xiangdong Yang ,  Guihua Liu ,  Xinfa Wang ,  Wei Hua ,  Jing Liu ,  Qing Yang ,  Yuanben Zheng

    发明人： Hanzhong Wang ,  Xiangdong Yang ,  Guihua Liu ,  Xinfa Wang ,  Wei Hua ,  Jing Liu ,  Qing Yang ,  Yuanben Zheng

    IPC分类号： A01H1/00 ,  C12N5/10 ,  C07H21/04 ,  A01H5/00

    CPC分类号： C12N15/8255 ,  C12N15/8282

    摘要： The use of a lignin monomer synthetic gene 4-CL encoding 4-coumarate: CoA ligase from Populus tomentosa in increasing Sclerotinia resistance and lodging resistance. The 4-CL gene is responsible for catalyzing various cinnamic acids and their derivatives to produce respective coenzyme A esters, involved in the synthesis of various downstream secondary products, and is the key gene in the regulation of lignin synthesis. Results of the study show that, increase of lignin content improves plant's resistance against pathogenic infection, enhances the stem strength of rape, and thus significantly improves the lodging resistance. Specifically, the result shows that lignin content of transgenic rape increases more than 10% compared to lignin content of recipient control; lesion extension area of transgenic plant leaves decreases around 30% compared to recipient control; and stem strength of transgenic plant increases up to around 20% compared to control.

    摘要翻译： 使用木质素单体合成基因4-CL编码4-香豆酸：来自山杨的CoA连接酶增加核盘菌素耐药性和耐倒伏性。 4-CL基因负责催化各种肉桂酸及其衍生物，产生各种辅酶A酯，涉及多种下游二级产物的合成，是木质素合成调控的关键基因。 研究结果表明，木质素含量的增加提高了植物对病原菌感染的抵抗力，提高了强奸的茎强度，从而显着提高了抗倒伏性。 具体来说，结果表明，转基因油菜的木质素含量与受体对照的木质素含量相比增加10％以上; 转基因植物叶片病变延伸面积比受体控制下降约30％; 转基因植物茎干强度与对照相比增加了约20％。