公开(公告)号：US20100285577A1

公开(公告)日：2010-11-11

申请号：US12672466

申请日：2008-08-07

申请人： Fariborz Izadyar ,  Jason Pachiarotti ,  Chad Maki ,  Thomas V. Ramos

发明人： Fariborz Izadyar ,  Jason Pachiarotti ,  Chad Maki ,  Thomas V. Ramos

IPC分类号： C12N5/07 ,  C12N5/074

CPC分类号： C12N5/0611 ,  C12N5/0609 ,  C12N2501/13 ,  C12N2502/13

摘要： Methods are provided for the isolation, characterization and propogation of germline cells stem cells from fetal and adult mammals. Additionally, isolated populations of germline cells having different phenotypes are disclosed wherein the subpopulations are capable of forming long-term cultures of multipotent or pluripotent cells or are capable of differentiating into mature germline cells and repopulating a sterile reproductive organ. The multipotent or pluripotent germline cells are also suitable for differentiation into tissue-specific somatic cells for therapeutic purposes.

摘要翻译： 提供了用于从胎儿和成年哺乳动物分离，表征和传播种系细胞干细胞的方法。 此外，公开了具有不同表型的分离的种系细胞，其中亚群能够形成多能或多能细胞的长期培养物，或能够分化成成熟种系细胞并重新填充无菌生殖器官。 多潜能或多能种系细胞也适用于分化为组织特异性体细胞用于治疗目的。