公开(公告)号：US20140189896A1

公开(公告)日：2014-07-03

申请号：US14105035

申请日：2013-12-12

申请人： Feng ZHANG ,  Le CONG ,  David Benjamin Turitz COX ,  Patrick HSU ,  Shuailiang LIN ,  Fei RAN ,  Randall Jeffrey PLATT ,  Neville Espi SANJANA ,  Luciano MARRAFFINI ,  David Olivier BIKARD ,  Wenyan JIANG

发明人： Feng ZHANG ,  Le CONG ,  David Benjamin Turitz COX ,  Patrick HSU ,  Shuailiang LIN ,  Fei RAN ,  Randall Jeffrey PLATT ,  Neville Espi SANJANA ,  Luciano MARRAFFINI ,  David Olivier BIKARD ,  Wenyan JIANG

IPC分类号： C12N15/85 ,  C12N15/74 ,  C12N15/70

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

摘要翻译： 本发明提供了用于操纵靶序列的序列和/或活性的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用这种载体的方法。 还提供了在真核细胞中引导CRISPR复合物形成的方法以及通过引入使用CRISPR / Cas系统的精确突变来选择特异性细胞的方法。

公开(公告)号：US20200263166A1

公开(公告)日：2020-08-20

申请号：US16095253

申请日：2017-04-19

申请人： Feng ZHANG ,  Bernd ZETSCHE ,  Winston YAN ,  Neville Espi SANJANA ,  Sara JONES ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Feng ZHANG ,  Bernd ZETSCHE ,  Winston YAN ,  Neville Espi SANJANA ,  Sara JONES

IPC分类号： C12N15/10 ,  C12N15/113 ,  C12N9/22 ,  C12N15/63

摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.