公开(公告)号：US20050112584A1

公开(公告)日：2005-05-26

申请号：US10718342

申请日：2003-11-20

申请人： Francisco Cifuentes ,  Meha Kapadia ,  Eric Leproust ,  Stephanie Fulmer-Smentek ,  Bill Peck

发明人： Francisco Cifuentes ,  Meha Kapadia ,  Eric Leproust ,  Stephanie Fulmer-Smentek ,  Bill Peck

IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： G16B25/00

摘要： Methods are disclosed for selecting a combination of nucleic acid sample pairs for evaluating the ability of an oligonucleotide probe to measure differential expression of genes. Differential gene expression experiments are conducted using (i) nucleic acid sample pairs and (ii) nucleic acid probes immobilized on a substrate, the probes representing a set of genes. The number of genes in the set is a portion of an expected number of genes in a sample. A nucleic acid sample pair combination is selected based on the members of the combination having a maximized number of genes from the set of genes that exhibit differential expression and a minimized number of the genes that do not exhibit differential expression.

摘要翻译： 公开了用于选择核酸样品对的组合以评估寡核苷酸探针以测量基因差异表达的能力的方法。 使用（i）核酸样品对和（ii）固定在底物上的核酸探针进行差异基因表达实验，探针代表一组基因。 该组中的基因数目是样品中预期基因数量的一部分。 基于组合的成员选择核酸样品对组合，其具有来自显示差异表达的基因组的最大数量的基因和不显示差异表达的基因的最小数目。