摘要:
Methods and devices for producing chemical arrays are provided. Aspects of methods include employing a dedicated wash fluid reaction chamber. In certain embodiments, aspects include contacting a surface with at least a deblocking reagent to produce a deblocked surface, washing the deblocked surface in a dedicated wash fluid reaction chamber, e.g., flow cell, and then contacting the washed surface with one or more reactive moieties in a spatially controlled manner. Also provided are devices configured for use in practicing the subject methods.
摘要:
A method and system for quantifying and correcting spatial-intensity trends for each channel of a microarray data set having one or more channels. The method and system of one embodiment of the present invention selects a set of features from each channel of the microarray data set. Based on the selected set of features, a surface is used to determine the intensities for all features in each channel of the microarray data set. Spatial-intensity trends within the microarray data set are quantified, based on the surface to the intensities for each channel of the microarray data set. After the surface has been determined, the spatial-intensity trend can be removed from the microarray data set.
摘要:
Methods and devices for fabricating a chemical array are provided. Embodiments include determining a chemical array layout in which each feature in the layout has a size that is chosen based on its composition and fabricating a chemical array according to the chemical array layout. In certain embodiments, at least two features of an array fabricated according to the subject methods are of different sizes. Embodiments also include chemical arrays having features of different sizes, e.g., fabricated according to the subject methods. Also provided are embodiments that include fluid deposition devices capable of fabricating chemical arrays having features of different sizes, e.g., for use in practicing the subject methods. Algorithms present on computer readable mediums for use in practicing the subject methods may also be provided in certain embodiments. Embodiments of the subject invention may also include systems and kits for use in practicing the subject methods.
摘要:
A generalized printhead control model is provided herein as a basis for configuring printhead control software that will operate any selected printhead or group of printheads. The subject model covers a hierarchy of classes for the attributes of software that controls the printheads in a system, such as for producing a biopolymer array.
摘要:
There are disclosed systems and methods in which a liquid dispensing head is positioned above the contact area of the device under test (DUT). A stream of liquid is dispensed from the head such that a continuous column of liquid extends from the head to the contact area of the test device. This column of liquid completes a circuit which allows current to flow thereby allowing for current measurement.
摘要:
The subject invention provides methods and devices for fabricating chemical arrays. Also provided are chemical arrays fabricated according to the subject methods, and methods of using chemical arrays produced according to the subject invention.
摘要:
Methods for fabricating chemical arrays are provided. Aspects of the invention include methods for providing solid activator in feature locations of a substrate surface. Aspects of the invention also include substrates having solid activator present in feature locations thereof, arrays produced from the substrates, methods for use of the arrays and kits that include the arrays.
摘要:
Methods of producing nucleic acid molecules using an in situ nucleic acid synthesis protocol are provided. The method can comprise contacting a substrate comprising an attached blocked nucleoside monomer or polymer with a deblocking fluid to remove the blocking group, thereby generating an unblocked attached nucleoside monomer or polymer; displacing the deblocking fluid from the substrate surface comprising the attached unblocked nucleoside monomer or polymer with a purging fluid; and reacting the attached unblocked nucleoside monomer or polymer with another blocked nucleoside monomer. Nucleic acid molecules produced by the methods are also provided and can be attached to or released from the substrate (e.g., provided in solution or in a lyophilized form).