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公开(公告)号:US5965422A
公开(公告)日:1999-10-12
申请号:US859106
申请日:1997-05-20
申请人: Fridolin Loffler , Quoc Khanh Nguyen , Erwin Schuster , Bruno Sprossler , Lutz Thomas , Sabine Wolf
发明人: Fridolin Loffler , Quoc Khanh Nguyen , Erwin Schuster , Bruno Sprossler , Lutz Thomas , Sabine Wolf
IPC分类号: C12N1/15 , C12N9/18 , C12N15/55 , C12N15/62 , C12N15/81 , C12S3/12 , C13K7/00 , C12N15/09 , C12N15/63
CPC分类号: C12N15/815 , C12N15/625 , C12N9/18 , C13K7/00 , C07K2319/02
摘要: This invention relates to a recombinant deoxyribonucleic acid (DNA) isolatable from Aspergillus, preferably Aspergillus foetidus, wherein it codes for a lysophospholipase (LPL) and has the nucleotide sequence for mature LPL stated in SEQ ID NO:1 or a nucleotide sequence derived therefrom, which hybridises under stringent conditions with the nucleotide sequence for mature LPL stated in SEQ ID NO:1. The invention also relates to vectors, to transformed host organisms and to processes for the production of LPL. The invention also provides enzyme products for the production of maltose syrup and products produced in this manner.
摘要翻译: 本发明涉及可从曲霉分离的重组脱氧核糖核酸(DNA),优选曲霉(Aspergillus foetidus),其编码溶血磷脂酶(LPL),并且具有SEQ ID NO:1所示的成熟LPL的核苷酸序列或由其衍生的核苷酸序列, 其在严格条件下与SEQ ID NO:1所述的成熟LPL的核苷酸序列杂交。 本发明还涉及载体,转化的宿主生物体和生产LPL的方法。 本发明还提供用于生产麦芽糖糖浆的酶产品和以这种方式生产的产品。