公开(公告)号：US20210355516A1

公开(公告)日：2021-11-18

申请号：US17038081

申请日：2020-09-30

Applicant: Fudan University

Inventor： Fener CHEN ,  Kejie ZHU ,  Zedu HUANG ,  Dang CHENG ,  Jiaqi WANG ,  Yuan TAO

IPC: C12P17/04 ,  C12N9/02 ,  C12N1/20 ,  C12N15/70

Abstract: An enzyme-catalyzed synthesis of (1S,5R)-bicyclolactone. A first genetically-engineered bacterium containing Baeyer-Villiger monooxygenase gene and a second genetically-engineered bacterium containing glucose dehydrogenase gene are constructed and then suspended with culture medium to prepare a first suspension and a second suspension, respectively. The first and second suspensions are centrifuged to respectively produce a first supernatant containing Baeyer-Villiger monooxygenase and a second supernatant containing glucose dehydrogenase, which are mixed. The mixed supernatant is then mixed with a raceme of a substituted bicyclo[3.2.0]-hept-2-en-6-one, a solvent, a hydrogen donor and a cofactor to perform an asymmetric Baeyer-Villiger oxidation to produce the (1S,5R)-bicyclolactone, where an amino acid sequence of the Baeyer-Villiger monooxygenase is shown in SEQ ID NO:1.