公开(公告)号：US20210054362A1

公开(公告)日：2021-02-25

申请号：US16955639

申请日：2018-12-21

申请人： G+FLAS LIFE SCIENCES

发明人： Jongjin PARK ,  Ji Young YOON ,  Sunmee CHOI ,  Mijin PARK ,  Slki PARK ,  Aiden Y. PARK ,  Jung Hyuk LEE ,  Junghak LIM ,  Dong Wook KIM ,  Sunghwa CHOE

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/113

摘要： The present disclosure provides compositions and methods for increasing mutation efficiency and homologous recombination rates of site-specific endonucleases. The compositions and methods comprise a chimeric polypeptide comprising a site-specific endonuclease or a domain thereof and a functional moiety. The current inventions relate to functional enhancement of the CRISPR-Cas enzymes. Disclosed herein include possible variants and their intended improvements.

公开(公告)号：US20230374478A1

公开(公告)日：2023-11-23

申请号：US18027598

申请日：2021-09-23

申请人： G FLAS LIFE SCIENCES

发明人： Jong Jin PARK ,  Jung Hyuk LEE

IPC分类号： C12N9/22 ,  C12N15/10

CPC分类号： C12N9/22 ,  C12N15/102 ,  C12N2310/20 ,  C07K2319/09

摘要： The present invention relates to a modified CRISPR-associated protein and a use thereof. More specifically, the present invention relates to a composition for genome editing comprising the modified CRISPR-associated protein and an enhancer, a method for genome editing using the same, and a method for producing a transformant by using the same. The modified CRISPR-associated protein according to the present invention is available as a nuclease having excellent indel efficiency in a CRISPR-Cas system, and exhibits excellent indel efficiency compared to conventional CRISPR-Cas systems, thus finding advantageous applications in genome editing.