公开(公告)号：US20200002728A1

公开(公告)日：2020-01-02

申请号：US16490623

申请日：2018-02-23

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Daniel Ivansson ,  Andreas Castan

IPC: C12N15/90 ,  C12P21/00 ,  C07K16/00

Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.

公开(公告)号：US11802297B2

公开(公告)日：2023-10-31

申请号：US16490623

申请日：2018-02-23

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Daniel Ivansson ,  Andreas Castan

IPC: C12N15/90 ,  C12N9/12 ,  C12P21/00 ,  C07K16/00

CPC classification number: C12N15/907 ,  C07K16/00 ,  C12P21/00 ,  C12N2510/02

Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.

公开(公告)号：US09983203B2

公开(公告)日：2018-05-29

申请号：US15108505

申请日：2015-02-12

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Daniel Ivansson ,  Camilla Estmer Nilsson ,  Therese Graner ,  Johan Ohman

IPC: G01N33/53 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/68 ,  C07H21/04 ,  G01N33/48 ,  G01N33/483 ,  C40B30/04

CPC classification number: G01N33/54333 ,  C12Q1/6804 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/6803 ,  G01N2333/79 ,  G01N2458/10

Abstract: Provided are methods for protein analysis in which proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection. The proteins to be analyzed may be provided as denatured protein samples in the presence of a denaturing agent.