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公开(公告)号：US10731118B2
公开(公告)日：2020-08-04
申请号：US15363562
申请日：2016-11-29
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Ralph Stankowski , Asa Lundstrom , Andreas Castan , Michael Miller , Yvette Klingberg
Abstract: Provided is a bioreactor assembly that includes a plurality of cultivation bags at least one flexible temperature control bag having a thermostating fluid, and a tray adapted to rock back and forth around at least one axis. The flexible temperature control bag is mounted on the tray and the cultivation bags are located on the flexible temperature control bag.
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公开(公告)号：US20170073624A1
公开(公告)日：2017-03-16
申请号：US15363562
申请日：2016-11-29
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Ralph Stankowski , Asa Lundstrom , Andreas Castan , Michael Miller , Yvette Klingberg
Abstract: The invention discloses a bioreactor assembly comprising: a) a plurality of cultivation bags; b) at least one flexible temperature control bag comprising a thermostating fluid, and; c) a tray adapted to rock back and forth around at least one axis, wherein the flexible temperature control bag is mounted on the tray and the cultivation bags are located on the flexible temperature control bag.
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公开(公告)号：US20200087605A1
公开(公告)日：2020-03-19
申请号：US16586272
申请日：2019-09-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Klaus Gebauer , Andreas Castan , Camilla Estmer-Nilsson , Thomas Falkman , Anders Wilen , Michael Miller , Ralph Stankowski
Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.
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公开(公告)号：US20200002728A1
公开(公告)日：2020-01-02
申请号：US16490623
申请日：2018-02-23
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Daniel Ivansson , Andreas Castan
Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.
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公开(公告)号：US20160194591A1
公开(公告)日：2016-07-07
申请号：US14910824
申请日：2013-08-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Andreas Castan , Klaus Gebauer
CPC classification number: C12M29/00 , C12M21/08 , C12M23/14 , C12M23/26 , C12M23/38 , C12M27/02 , C12M27/16 , C12M29/14 , C12N1/16 , C12N1/20 , C12N5/04 , C12N5/0601 , C12N5/0602
Abstract: The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.
Abstract translation: 本发明公开了一种具有容器的生物反应器，该容器限定内容积，搅拌装置和至少一个加料管，其中加料管中的输送孔位于内体积内，止回阀布置在输送孔附近，以允许 流体在从加料管的方向流入容器的内部容积并阻止反向流动。
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Patent Agency Ranking
公开(公告)号：US10760041B2
公开(公告)日：2020-09-01
申请号：US14915808
申请日：2014-08-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Karl Liderfelt , Eric Faldt , Andreas Castan
Abstract: The invention discloses a flexible bag for cell cultivation, comprising a cultivation compartment and at least one dialysis compartment, wherein the dialysis compartment(s) is/are delimited from the cultivation compartment by one or more dialysis membranes and is/are fluidically connected to a first and a second port in the bag.
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公开(公告)号：US10450540B2
公开(公告)日：2019-10-22
申请号：US15306691
申请日：2015-04-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Klaus Gebauer , Andreas Castan , Camilla Estmer-Nilsson , Thomas Falkman , Anders Wilen , Michael Miller , Ralph Stankowski
Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.
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公开(公告)号：US20180251790A1
公开(公告)日：2018-09-06
申请号：US15907470
申请日：2018-02-28
Applicant: GE HEALTHCARE BIO-SCIENCES AB
Inventor： Daniel Leif Ivansson , Andreas Castan
CPC classification number: C12N15/907 , C12N9/22 , C12N15/11 , C12N2310/20 , C12N2800/80
Abstract: The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells.The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.
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公开(公告)号：US20170044477A1
公开(公告)日：2017-02-16
申请号：US15306691
申请日：2015-04-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Klaus Gebauer , Andreas Castan , Camilla Estmer-Nilsson , Thomas Falkman , Anders Wilen , Michael Miller , Ralph Stankowski
CPC classification number: C12M23/14 , C12M23/26 , C12M23/34 , C12M23/58 , C12M27/16 , C12M41/24 , C12Q3/00
Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.
Abstract translation: 本发明公开了一种用于培养细胞的柔性袋组件，包括形成多个培养隔室的一个或多个袋，其中至少第一栽培室中的排出口适于在打开第二培养室时与第二培养室流体连接 阀门装置。 它还公开了一种生物反应器，其中袋组件安装在摆动托盘上，以及在组件中培养细胞的方法。
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公开(公告)号：US11802297B2
公开(公告)日：2023-10-31
申请号：US16490623
申请日：2018-02-23
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Daniel Ivansson , Andreas Castan
CPC classification number: C12N15/907 , C07K16/00 , C12P21/00 , C12N2510/02
Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.
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