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公开(公告)号：US20160282318A1
公开(公告)日：2016-09-29
申请号：US15066429
申请日：2016-03-10
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Gunnar Malmquist , Martin Hall , Nils Stafstrom
Abstract: The invention discloses a method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base. The method comprises the steps of:a) providing a chromatography system with a chromatography column and a UV detector downstream of the chromatography column; b) providing UV extinction coefficients at a wavelength λ for the acid form and the base form of the UV-absorbing component(s) and a dissociation constant for the component(s), c) obtaining a chromatogram with the UV detector at wavelength λ using the buffer under a set of conditions where pH and/or conductivity is varied between these conditions, d) for a plurality of said conditions calculating the concentrations of the acid and base forms using the dissociation constant(s) and values for pH and conductivity values, and e) calculating UV absorption values of the acid and base forms from the concentrations and UV extinction coefficients under the conditions in question and subtracting the UV absorption values from the chromatogram.
Abstract translation: 一种用于使用具有至少一种UV吸收组分（其为弱酸或弱碱）的缓冲液获得的色谱图中校正基线的方法，包括以下步骤：a）提供具有色谱柱和UV检测器的色谱系统 色谱柱下游; b）为UV形吸收组分的酸形式和碱性形式提供波长为λ的紫外消光系数和组分的解离常数; c）使用UV检测器在波长λ下使用缓冲液在这些条件下pH和/或电导率变化的一组条件下获得色谱图; d）对于多个所述条件，使用解离常数和pH和电导率值计算酸和碱形式的浓度，以及e）从浓度和UV计算酸和碱形式的UV吸收值 在所述条件下的消光系数，并从色谱图中减去紫外吸收值。 酸和碱形式的浓度可以使用Debye-Huckel方程计算。 还要求的是用于执行如上所述的方法的色谱系统。
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公开(公告)号：US10598640B2
公开(公告)日：2020-03-24
申请号：US15066429
申请日：2016-03-10
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Gunnar Malmquist , Martin Hall , Nils Stafstrom
Abstract: The invention discloses a method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base. The method comprises the steps of:a) providing a chromatography system with a chromatography column and a UV detector downstream of the chromatography column;b) providing UV extinction coefficients at a wavelength λ for the acid form and the base form of the UV-absorbing component(s) and a dissociation constant for the component(s),c) obtaining a chromatogram with the UV detector at wavelength λ using the buffer under a set of conditions where pH and/or conductivity is varied between these conditions,d) for a plurality of said conditions calculating the concentrations of the acid and base forms using the dissociation constant(s) and values for pH and conductivity values, ande) calculating UV absorption values of the acid and base forms from the concentrations and UV extinction coefficients under the conditions in question and subtracting the UV absorption values from the chromatogram.
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公开(公告)号：US20190336886A1
公开(公告)日：2019-11-07
申请号：US16474455
申请日：2017-12-22
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Key Hyckenberg , Mikael Johan Helmer Eugen Berg , Helena Skoglar , Gunnar Malmquist , Linda Mathiasson , Lars Henning Ivar Mattsson , Sara Sendabo
Abstract: The present invention relates to a method for controlling a bioprocess purification system comprising a continuous chromatography configured to operate with at least three columns and configured for cyclic operation, wherein the continuous purification is performed on a sample comprising a target product having desired characteristics. The method comprises detecting (82) at least one parameter indicative of characteristics of the target product, performing (83) real time trend analysis of each detected at least one parameter to identify a deviation from the desired characteristics of the target product, and controlling (84) the bioprocess purification system to meet the desired characteristics based on the identified deviation, whereby the target characteristics is within a pre-determined range.
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