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公开(公告)号：US20170067031A1
公开(公告)日：2017-03-09
申请号：US15355675
申请日：2016-11-18
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Patrik Adielsson , Tobias E. Soderman , Anna Akerblom
CPC classification number: C12N7/00 , B01D15/3847 , C12N2760/16151
Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.
Abstract translation: 本发明涉及一种从样品中去除内毒素的方法，包括以下步骤：将包含一种或多种靶分子的样品与包含珠的色谱介质组合，所述珠粒具有由能够结合内毒素的配体功能化的内部多孔核心和 没有官能团的外部多孔层和足够小以从靶芯排出靶分子的孔径; 并从培养基中收集样品，其中样品包含与去除前相比至少75％以上，优选90％以下的内毒素水平，并且目标分子的产率为至少75％。
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公开(公告)号：US09580692B2
公开(公告)日：2017-02-28
申请号：US14380477
申请日：2013-02-27
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Patrik Adielsson , Tobias E. Soderman , Anna Akerblom
IPC: C12N7/02 , B01D15/36 , C12N7/00 , C07K1/20 , B01D15/38 , B01J20/24 , B01J20/285 , B01J20/286
CPC classification number: C12N7/00 , B01D15/363 , B01D15/3847 , B01J20/24 , B01J20/285 , B01J20/286 , C07K1/20 , C12N2760/16151
Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.
Abstract translation: 本发明涉及一种从样品中去除内毒素的方法，包括以下步骤：将包含一种或多种靶分子的样品与包含珠的色谱介质组合，所述珠粒具有由能够结合内毒素的配体功能化的内部多孔核心和 没有官能团的外部多孔层和足够小以从靶芯排出靶分子的孔径; 并从培养基中收集样品，其中样品包含与去除前相比至少75％以上，优选90％以下的内毒素水平，并且目标分子的产率为至少75％。
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公开(公告)号：US10041050B2
公开(公告)日：2018-08-07
申请号：US15355675
申请日：2016-11-18
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Patrik Adielsson , Tobias E. Soderman , Anna Akerblom
Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.
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