公开(公告)号：US20190218265A1

公开(公告)日：2019-07-18

申请号：US16328058

申请日：2017-09-05

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Sophia Hober ,  Sara Kanje ,  Johan Nilvebrant

IPC: C07K14/47 ,  C07K1/22

CPC classification number: C07K14/4728 ,  C07K1/22 ,  C07K14/31 ,  C07K2319/20 ,  C07K2319/30 ,  C07K2319/705

Abstract: The present invention is within the field of protein engineering and purification. The invention relates to a target-binding polypeptide mutant of an IgG binding polypeptide, such as Protein A, Protein G, Protein L or Protein M, comprising a metal binding motif. More closely the invention relates to an Fc binding ligand comprising an engineered protein based on the Protein A derived Z domain, to which a calcium binding EF-loop has been introduced.