公开(公告)号：US20160238592A1

公开(公告)日：2016-08-18

申请号：US14621715

申请日：2015-02-13

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Arunkumar Natarajan ,  Anup Sood ,  Lakshmi Sireesha Kaanumalle ,  Christina Lowes

IPC: G01N33/536 ,  G01N1/30 ,  G01N33/58

CPC classification number: G01N33/536 ,  G01N1/30 ,  G01N21/6428 ,  G01N33/582 ,  G01N2001/302

Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.

Abstract translation: 提供了包括使用光活化化学漂白来检测生物样品中的多个靶的方法。 所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一个探针结合到样品中存在的一个或多个靶，并观察来自探针的信号。 该方法还包括使包含结合的探针的样品与阳离子或两性离子的硼酸盐化合物接触并照射样品的步骤，从而引发通过光活化的化学漂白基本上灭活探针的光反应。 该方法还包括将至少一个探针结合到样品中存在的一个或多个靶，并观察来自探针的信号的步骤。 结合，观察和漂白的过程可以迭代地重复。

公开(公告)号：US10101322B2

公开(公告)日：2018-10-16

申请号：US14621715

申请日：2015-02-13

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Arunkumar Natarajan ,  Anup Sood ,  Lakshmi Sireesha Kaanumalle ,  Christina Lowes

IPC: G01N33/536 ,  G01N33/58 ,  G01N21/64 ,  G01N1/30

Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.