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1.
公开(公告)号:US20190080146A1
公开(公告)日:2019-03-14
申请号:US15703835
申请日:2017-09-13
Applicant: General Electric Company
Inventor: Alberto Santamaria-Pang , Qing Li , Yunxia Sui , Dmitry Vladimirovich Dylov , Christopher James Sevinsky , Michael E. Marino , Michael J. Gerdes , Daniel Eugene Meyer , Fiona Ginty , Anup Sood
CPC classification number: G06K9/00147 , G06T7/0012 , G06T7/33 , G06T2207/10024 , G06T2207/10056 , G06T2207/20104 , G06T2207/30024
Abstract: The subject matter of the present disclosure generally relates to techniques for image analysis. In certain embodiments, various morphological or intensity-based features as well as different thresholding approaches may be used to segment the subpopulation of interest and classify object in the images.
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公开(公告)号:US10101322B2
公开(公告)日:2018-10-16
申请号:US14621715
申请日:2015-02-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor: Arunkumar Natarajan , Anup Sood , Lakshmi Sireesha Kaanumalle , Christina Lowes
IPC: G01N33/536 , G01N33/58 , G01N21/64 , G01N1/30
Abstract: Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.
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公开(公告)号:US09708349B2
公开(公告)日:2017-07-18
申请号:US14621776
申请日:2015-02-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor: Arunkumar Natarajan , Anup Sood , Lakshmi Sireesha Kaanumalle
CPC classification number: C07F5/02
Abstract: A borate compound for use as photoinduced chemical bleaching reagent is disclosed having improved bleaching performance under certain conditions, compared to neutral borates. The compounds are comprised of Formula I; R1R2R3R4BXa I where each R1, R2, and R3 and R4 is, independently, an alkyl, alkylaryl, or arylalkyl, with the proviso that at least one of R1, R2, and R3 and R4 is an alkyl group and at least two of R1, R2, R3 and R4 group is positively charged; X is an anion; and a equals 1, 2, or 3.
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公开(公告)号:US09322051B2
公开(公告)日:2016-04-26
申请号:US14047233
申请日:2013-10-07
Applicant: GENERAL ELECTRIC COMPANY
Inventor: Anup Sood , Arunkumar Natarajan , Michael James Rishel , Michael John Gerdes
IPC: C12Q1/68 , G01N33/68 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/543
CPC classification number: C12Q1/6825 , C12Q2523/319 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/6854
Abstract: Disclosed are high throughput methods of probing multiple targets in a biological sample where the recurrent time-consuming antibody incubation steps and individual signal modification and activation steps are replaced by simultaneous hybridization of biomarkers and sequential detection by combining signal removal and activation into a single step. Also disclosed are images obtained by such methods.
Abstract translation: 公开了探测生物样品中多个靶标的高通量方法,其中复发耗时的抗体孵育步骤和单独的信号修饰和激活步骤被生物标志物的同时杂交和通过将信号去除和活化组合到单个步骤中的顺序检测来代替。 还公开了通过这种方法获得的图像。
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5.
公开(公告)号:US09176032B2
公开(公告)日:2015-11-03
申请号:US13786747
申请日:2013-03-06
Applicant: General Electric Company
Inventor: Anup Sood , Kevin Bernard Kenny , Arunkumar Natarajan , Lakshmi Sireesha Kaanumalle
IPC: G01N33/53 , G01N33/532 , G01N33/533 , G01N21/76 , G01N1/30 , G01N33/58 , G01N21/64
CPC classification number: G01N1/30 , G01N21/64 , G01N21/6428 , G01N33/582
Abstract: Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample, observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated.
Abstract translation: 提供了包括在H&E染色的生物样品中使用探测多个靶标的方法。 所述方法包括以下步骤:提供含有多个靶标的苏木精和伊红染色的生物样品,观察样品,除去苏木精并通过洗涤样品部分除去曙红,使样品与硼酸盐接触,并照射样品以除去 残余曙红荧光。 该方法还包括任选地执行将至少一个探针结合至样品的一个或多个靶的附加步骤,观察来自探针的信号并使样品与漂白剂接触。 结合,观察和漂白的过程可以迭代地重复。
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公开(公告)号:US20130178392A1
公开(公告)日:2013-07-11
申请号:US13786747
申请日:2013-03-06
Applicant: General Electric Company
Inventor: Anup Sood , Kevin Bernard Kenny , Arunkumar Natarajan , Lakshmi Sireesha Kaanumalle
IPC: G01N1/30
CPC classification number: G01N1/30 , G01N21/64 , G01N21/6428 , G01N33/582
Abstract: Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample ,observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated.
Abstract translation: 提供了包括在H&E染色的生物样品中使用探测多个靶标的方法。 所述方法包括以下步骤:提供含有多个靶标的苏木精和伊红染色的生物样品,观察样品,除去苏木精并通过洗涤样品部分除去曙红,使样品与硼酸盐接触,并照射样品以除去 残余曙红荧光。 该方法还包括任选地执行将至少一个探针结合至样品的一个或多个靶的附加步骤,观察来自探针的信号并使样品与漂白剂接触。 结合,观察和漂白的过程可以迭代地重复。
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7.
公开(公告)号:US10423820B2
公开(公告)日:2019-09-24
申请号:US15703835
申请日:2017-09-13
Applicant: General Electric Company
Inventor: Alberto Santamaria-Pang , Qing Li , Yunxia Sui , Dmitry Vladimirovich Dylov , Christopher James Sevinsky , Michael E. Marino , Michael J. Gerdes , Daniel Eugene Meyer , Fiona Ginty , Anup Sood
Abstract: The subject matter of the present disclosure generally relates to techniques for image analysis. In certain embodiments, various morphological or intensity-based features as well as different thresholding approaches may be used to segment the subpopulation of interest and classify object in the images.
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公开(公告)号:US20180057791A1
公开(公告)日:2018-03-01
申请号:US15245584
申请日:2016-08-24
Applicant: General Electric Company
Inventor: Ernest William Kovacs , Anup Sood , Reginald Donovan Smith , Evelina Roxana Loghin , Padmaparna Chadhuri , Vandana Keskar , Chrystal Mae Chadwick , Martin James Brown
IPC: C12N5/0783 , C07K16/28
CPC classification number: C12N5/0636 , C07K16/2809 , C07K16/2818 , C12N2501/51 , C12N2501/515
Abstract: Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.
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公开(公告)号:US20170122872A1
公开(公告)日:2017-05-04
申请号:US15299085
申请日:2016-10-20
Applicant: General Electric Company
Inventor: Anup Sood , ARUNKUMAR NATARAJAN , Dmitry Dylov , Lakshmi Sireesha Kaanumalle , Elizabeth Mary McDonough
IPC: G01N21/64 , G01N33/574 , G01N33/483
CPC classification number: G01N21/6458 , G01N21/6428 , G01N33/4833 , G01N33/57407 , G01N2021/6439 , G01N2333/70503 , G01N2333/91215
Abstract: The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of: a) providing a biological microscopy sample; b) irradiating the sample with visible light, wherein the visible light has a spectrum such that at least 50% of the light intensity emanates from a narrow wavelength interval within the visible range. The invention also discloses a method for autofluorescence reduction with triplet sensitizers irradiated with visible light.
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公开(公告)号:US20170007636A1
公开(公告)日:2017-01-12
申请号:US15219419
申请日:2016-07-26
Applicant: General Electric Company
Inventor: Praveena Mohan , Anup Sood , James Edward Rothman , John Donald Burczak , Hae Won Lim , Lisa Anne Lowery
IPC: A61K31/713 , C12N15/113 , A61M37/00 , A61K47/48 , A61K47/06 , A61K41/00 , A61K9/00
CPC classification number: A61K31/713 , A61K9/0019 , A61K41/0028 , A61K47/06 , A61K47/554 , A61K47/64 , A61K47/643 , A61K47/6925 , A61M37/0092 , A61M2037/0007 , C12N15/111 , C12N15/1136 , C12N15/87 , C12N2310/14 , C12N2310/3515 , C12N2320/32
Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.
Abstract translation: 本发明涉及一种微泡复合物,其包含具有外壳的微泡,所述微泡包含天然和包封全氟化碳气体的变性白蛋白的混合物,治疗剂,具有连接于治疗剂的一端的双功能连接体,另一端连接于配体 并且其中配体通过疏水相互作用结合到微泡的另一个壳体。 还包括用于将上述微泡复合物递送到组织靶的方法。
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