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    ISOTHERMAL AMPLIFICATION UNDER LOW SALT CONDITION
    3.
    发明申请
    ISOTHERMAL AMPLIFICATION UNDER LOW SALT CONDITION 有权
    低盐条件下的同位素放大

    公开(公告)号：US20150275282A1

    公开(公告)日：2015-10-01

    申请号：US14225887

    申请日：2014-03-26

    Applicant: General Electric Company

    Inventor： Ryan Charles Heller ,  John Richard Nelson ,  Paresh Lakhubhai Patel ,  Alison Myfanwy Wakefield ,  Stephen James Capper

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12P19/34 ,  C12Q1/6844 ,  C12Q2527/107 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2531/119

    Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.

    Abstract translation: 本文提供了使用靶核酸模板的等温核酸扩增的方法和试剂盒; 包含具有链置换活性的DNA聚合酶，脱氧核糖核苷三磷酸（dNTP）混合物，具有3'端和5'末端的引物，分子拥塞试剂和用于扩增靶核酸模板的缓冲溶液的反应混合物 。 缓冲溶液维持反应混合物的低盐浓度，其中盐浓度导致底漆的熔融温度（Tm）低于反应温度至少10℃。 在等温条件下进行扩增。

    Isothermal amplification under low salt condition
    4.
    发明授权
    Isothermal amplification under low salt condition 有权

    公开(公告)号：US10597691B2

    公开(公告)日：2020-03-24

    申请号：US15408509

    申请日：2017-01-18

    Applicant: General Electric Company

    Inventor： Ryan Charles Heller ,  John Richard Nelson ,  Paresh Lakhubhai Patel ,  Alison Myfanwy Wakefield ,  Stephen James Capper

    IPC: C12P19/34 ,  C12Q1/6844

    Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.

    Isothermal amplification under low salt condition
    6.
    发明授权
    Isothermal amplification under low salt condition 有权
    低盐条件下的等温放大

    公开(公告)号：US09587263B2

    公开(公告)日：2017-03-07

    申请号：US14225887

    申请日：2014-03-26

    Applicant: General Electric Company

    Inventor： Ryan Charles Heller ,  John Richard Nelson ,  Paresh Lakhubhai Patel ,  Alison Myfanwy Wakefield ,  Stephen James Capper

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12P19/34 ,  C12Q1/6844 ,  C12Q2527/107 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2531/119

    Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.

    Abstract translation: 本文提供了使用靶核酸模板的等温核酸扩增的方法和试剂盒; 包含具有链置换活性的DNA聚合酶，脱氧核糖核苷三磷酸（dNTP）混合物，具有3'端和5'末端的引物，分子拥塞试剂和用于扩增靶核酸模板的缓冲溶液的反应混合物 。 缓冲溶液维持反应混合物的低盐浓度，其中盐浓度导致底漆的熔融温度（Tm）低于反应温度至少10℃。 在等温条件下进行扩增。

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