公开(公告)号：US10030241B2

公开(公告)日：2018-07-24

申请号：US15150806

申请日：2016-05-10

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Patrick McCoy Spooner ,  Peter James Tatnell ,  Jeffrey Kenneth Horton ,  John Richard Nelson ,  Michael John Gerdes ,  Suzana Kiel ,  Ralf Lenigk ,  Alexander Schenk ,  Wei Sun

IPC: C12N15/10 ,  G01N33/53 ,  C12Q1/6804 ,  G01N27/447 ,  G01N33/68 ,  C12Q1/6888

Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

公开(公告)号：US20160289666A1

公开(公告)日：2016-10-06

申请号：US15150806

申请日：2016-05-10

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Patrick McCoy Spooner ,  Peter James Tatnell ,  Jeffrey Kenneth Horton ,  John Richard Nelson ,  Michael John Gerdes ,  Suzana Kiel ,  Ralf Lenigk ,  Alexander Schenk ,  Wei Sun

IPC: C12N15/10 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/53

CPC classification number: C12N15/1013 ,  C12N15/101 ,  C12Q1/6804 ,  C12Q1/6888 ,  C12Q2600/124 ,  G01N27/44743 ,  G01N27/44747 ,  G01N33/5308 ,  G01N33/689 ,  C12Q2522/101

Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

Abstract translation: 本发明一般涉及从生物样品中或在生物样品中捕获精子核酸的方法和试剂盒。 在一个实施方案中，该方法包括使样品与裂解溶液接触，其具有鱼精蛋白-DNA复合物，以裂解细胞并施用鱼精蛋白特异性抗体。 这导致鱼精蛋白特异性抗体结合鱼精蛋白-DNA以形成可被捕获，纯化或检测的复合物。 还提供了用于实施所公开的方法的套件。

公开(公告)号：US10443094B2

公开(公告)日：2019-10-15

申请号：US15419560

申请日：2017-01-30

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Ryan Charles Heller ,  John Richard Nelson ,  Paresh Lakhubhai Patel ,  Alison Myfanwy Wakefield ,  Stephen James Capper ,  Peter James Tatnell ,  Jeffrey Kenneth Horton

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12P19/34

Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.

公开(公告)号：US20170137874A1

公开(公告)日：2017-05-18

申请号：US15419560

申请日：2017-01-30

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Ryan Charles Heller ,  John Richard Nelson ,  Paresh Lakhubhai Patel ,  Alison Myfanwy Wakefield ,  Stephen James Capper ,  Peter James Tatnell ,  Jeffrey Kenneth Horton

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12P19/34 ,  C12Q2527/107 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2531/119

Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.