公开(公告)号：US09839686B2

公开(公告)日：2017-12-12

申请号：US14612111

申请日：2015-02-02

Applicant: GlaxoSmithKline Biologicals SA

Inventor： Francesco Berti ,  Paolo Costantino ,  Maria Rosaria Romano

IPC: A61K39/385 ,  A61K39/085 ,  A61K31/70 ,  A61K47/48 ,  A61K47/64 ,  A61K39/00

CPC classification number: A61K39/385 ,  A61K39/085 ,  A61K47/6415 ,  A61K47/646 ,  A61K2039/6037 ,  A61K2039/627 ,  Y10S424/831 ,  Y10S530/807

Abstract: The invention provides a process for preparing a conjugate of a S. aureus type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.

公开(公告)号：US10736959B2

公开(公告)日：2020-08-11

申请号：US15809524

申请日：2017-11-10

Applicant: GLAXOSMITHKLINE BIOLOGICALS, SA

Inventor： Francesco Berti ,  Paolo Costantino ,  Maria Rosaria Romano

IPC: A61K47/64 ,  A61K39/085 ,  A61K39/385 ,  A61K39/00

Abstract: The invention provides a process for preparing a conjugate of a S. aureus type 5 or type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.

公开(公告)号：US10172928B2

公开(公告)日：2019-01-08

申请号：US15536132

申请日：2015-12-17

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco Berti ,  Paolo Costantino ,  Maria Rosaria Romano

IPC: G01N1/18 ,  A61K39/09 ,  C08B37/00

Abstract: A purification method for the capsular polysaccharide of type II GBS in which the capsular polysaccharide is filtered using a membrane with a cut-off of less than 30 kDa.

公开(公告)号：US09441004B2

公开(公告)日：2016-09-13

申请号：US14714097

申请日：2015-05-15

Applicant: GlaxoSmithKline Biologicals SA

Inventor： Paolo Costantino ,  Maria Rosaria Romano ,  Francesco Berti

IPC: A61K47/48 ,  A61K39/00 ,  C07H1/08 ,  C12P19/04 ,  A61K39/085

CPC classification number: C07H1/08 ,  A61K39/085 ,  A61K2039/55505 ,  A61K2039/6037 ,  C12P19/04

Abstract: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g. to remove low molecular weight contaminants; anion exchange chromatography, e.g. to remove residual protein; and concentration.

Abstract translation: 本发明提供了一种从金黄色葡萄球菌5型或8型细胞中释放荚膜多糖的方法，包括用酸处理细胞的步骤。 本发明还提供了一种从包括该方法的金黄色葡萄球菌5型或8型细胞中纯化荚膜多糖的方法。 其他处理步骤可以包括在该方法中，例如酶处理，例如， 去除核酸，蛋白质和/或肽聚糖污染物; 渗滤，例如 去除低分子量污染物; 阴离子交换层析，例如 去除残留蛋白质; 和集中。