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    Conjugation of Streptococcal capsular saccharides
    2.
    发明授权
    Conjugation of Streptococcal capsular saccharides 有权

    公开(公告)号：US10188719B2

    公开(公告)日：2019-01-29

    申请号：US15592339

    申请日：2017-05-11

    Applicant: GlaxoSmithKline Biologicals SA

    Inventor： Francesco Berti

    IPC: A61K39/09 ,  C07K14/315 ,  A61K39/385 ,  A61K47/64 ,  A61K39/00

    Abstract: Three conjugation methods for use with the capsular saccharide of Streptococcus agalactiae. In the first method, reductive amination of oxidized sialic acid residue side chains is used, but the aldehyde groups are first aminated, and then the amine is coupled to a carrier via a linker. In the second method, sialic acid residues and/or N-acetyl-glucosamine residues are de-N-acetylated to give amine groups, and the amine groups are coupled to a carrier protein via a linker. In the third method, linkage is via galactose residues in the capsular saccharide rather than sialic acid residues, which can conveniently be achieved using galactose oxidase.

    Method of detecting the presence of an antibody in a sample
    6.
    发明授权
    Method of detecting the presence of an antibody in a sample 有权
    检测样品中抗体的存在的方法

    公开(公告)号：US09562894B2

    公开(公告)日：2017-02-07

    申请号：US14363383

    申请日：2012-12-12

    Applicant: GlaxoSmithKline Biologicals SA

    Inventor： Francesco Berti ,  Carl E. Frasch

    IPC: G01N33/569 ,  G01N33/543 ,  C07K16/12 ,  G01N33/68 ,  A61K39/40 ,  A61K39/00

    CPC classification number: G01N33/54306 ,  A61K39/40 ,  A61K2039/6037 ,  A61K2039/627 ,  C07K16/1275 ,  G01N33/6854 ,  G01N2400/40

    Abstract: Methods for detecting in a sample the presence of an antibody to a conjugate of an antigen associated with a first carrier by a first association are disclosed. The method comprises contacting a conjugate of the antigen associated with a second carrier by a second association with said sample under conditions that allow binding of the antibody to the antigen; and introducing an agent to detect the presence of the antibody bound to said antigen. The first association and the second association are covalent associations and the first association is different from the second association. Also provided are kits, multiwell plates and conjugates that are useful in the method and further uses of the method. Also provided is a method of releasing a batch of a vaccine comprising a conjugate of an antigen associated with a first carrier by a first association and antibodies useful in this method.

    Abstract translation: 公开了通过第一关联在样品中检测与第一载体相关的抗原的缀合物的抗体的存在的方法。 该方法包括在允许抗体与抗原结合的条件下，将与第二载体相关的抗原的缀合物与所述样品的第二结合物接触; 并引入试剂以检测与所述抗原结合的抗体的存在。 第一关联和第二关联是共价关联，第一关联与第二关联不同。 还提供了可用于该方法的方法和进一步使用的试剂盒，多孔板和缀合物。 还提供了一种释放一批疫苗的方法，该疫苗包含通过第一缔合与第一载体相关的抗原的缀合物和在该方法中有用的抗体。

    Conjugation of Staphylococcus aureus type 5 and type 8 capsular polysaccharides
    8.
    发明授权
    Conjugation of Staphylococcus aureus type 5 and type 8 capsular polysaccharides 有权

    公开(公告)号：US10736959B2

    公开(公告)日：2020-08-11

    申请号：US15809524

    申请日：2017-11-10

    Applicant: GLAXOSMITHKLINE BIOLOGICALS, SA

    Inventor： Francesco Berti ,  Paolo Costantino ,  Maria Rosaria Romano

    IPC: A61K47/64 ,  A61K39/085 ,  A61K39/385 ,  A61K39/00

    Abstract: The invention provides a process for preparing a conjugate of a S. aureus type 5 or type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.

    Purification of staphylococcus aureus type 8 capsular saccharides
    10.
    发明授权
    Purification of staphylococcus aureus type 8 capsular saccharides 有权
    金黄色葡萄球菌8型荚膜糖的纯化

    公开(公告)号：US09441004B2

    公开(公告)日：2016-09-13

    申请号：US14714097

    申请日：2015-05-15

    Applicant: GlaxoSmithKline Biologicals SA

    Inventor： Paolo Costantino ,  Maria Rosaria Romano ,  Francesco Berti

    IPC: A61K47/48 ,  A61K39/00 ,  C07H1/08 ,  C12P19/04 ,  A61K39/085

    CPC classification number: C07H1/08 ,  A61K39/085 ,  A61K2039/55505 ,  A61K2039/6037 ,  C12P19/04

    Abstract: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g. to remove low molecular weight contaminants; anion exchange chromatography, e.g. to remove residual protein; and concentration.

    Abstract translation: 本发明提供了一种从金黄色葡萄球菌5型或8型细胞中释放荚膜多糖的方法，包括用酸处理细胞的步骤。 本发明还提供了一种从包括该方法的金黄色葡萄球菌5型或8型细胞中纯化荚膜多糖的方法。 其他处理步骤可以包括在该方法中，例如酶处理，例如， 去除核酸，蛋白质和/或肽聚糖污染物; 渗滤，例如 去除低分子量污染物; 阴离子交换层析，例如 去除残留蛋白质; 和集中。

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