公开(公告)号：US09024008B2

公开(公告)日：2015-05-05

申请号：US13808139

申请日：2010-07-07

申请人： Gabor Kiss ,  Janos Kiss ,  Katalin Sztancsik Ambrusné Kovács ,  Georgina Bernath

发明人： Gabor Kiss ,  Janos Kiss ,  Katalin Sztancsik Ambrusné Kovács ,  Georgina Bernath

IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1003 ,  C07H21/00 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q2523/32

摘要： Procedure for the specific isolation of total DNA content of bacterial germs of different samples, in the course of which the cells are lysated, the DNA content of the lysate is bound selectively, it is washed and then the desalinated linear polymer nucleic acid is eluted from the binding surface in an aqueous solution. Before cell lysis the nonviable bacterial cells are separated from the viable cells on the basis of their different cell surface physical-chemical characteristics, the viable cells of the sample are kept and then lysated using a mechanical and/or enzymatic, favorably lysozyme enzymatic method. After this exclusively double-stranded DNA deriving from the lysate of viable cells is bound on a —SiO2—TiO2- matrix containing chemically activated —OH and dodecylamine groups, and after washing it, the desalinated linear polymer nucleic acid is eluted in an aqueous solution.

摘要翻译： 特异性分离不同样品的细菌细菌的总DNA含量的步骤，在细胞裂解过程中，裂解物的DNA含量选择性地结合，洗涤，然后脱盐的线性聚合物核酸从 在水溶液中的结合表面。 在细胞裂解之前，根据不同的细胞表面物理化学特性，将不可维护的细菌细胞从活细胞中分离出来，保存样品的活细胞，然后使用机械和/或酶促的溶菌酶酶法进行裂解。 之后，将衍生自活细胞裂解物的纯双链DNA结合在含有化学活化的-OH和十二烷基胺基团的-SiO 2 -TiO 2基质上，并且在洗涤之后，脱盐的线性聚合物核酸在水溶液 。