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公开(公告)号:US20140287022A1
公开(公告)日:2014-09-25
申请号:US14113707
申请日:2012-04-25
申请人: Gary Fujii , William A. Ernst , Jill Adler-Moore , Linda Vician
发明人: Gary Fujii , William A. Ernst , Jill Adler-Moore , Linda Vician
IPC分类号: A61K9/127 , A61K45/06 , A61K39/145
CPC分类号: A61K9/127 , A61K9/1277 , A61K39/12 , A61K39/145 , A61K45/06 , A61K2039/543 , A61K2039/55555 , A61K2039/55572 , A61K2039/6031 , C07K2319/03 , C07K2319/35 , C12N2760/16134
摘要: The present invention relates to liposomal vaccine compositions, methods for the manufacture thereof, and methods for the use thereof to stimulate an immune response in an animal. These compositions comprise liposomes formed from dimyristoylphosphatidylcholine (“DMPC”); either dimyristoylphosphatidylglycerol (“DMPG”) or dimyristoyltrimethylammonium propane (“DMTAP”) or both DMPC and DMTAP; and at least one sterol; and an antigenic polypeptide comprising a first polypeptide sequence, and a second polypeptide sequence heterologous the first polypeptide sequence which comprises a transmembrane sequence from a membrane protein, said transmembrane sequence having a number of residues sufficient to cross a lipid bilayer, at least nine contiguous residues of which are predicted to form an alpha helix having a hydrophobicity score of about 0.7 or greater.
摘要翻译: 本发明涉及脂质体疫苗组合物,其制备方法及其用于刺激动物免疫应答的方法。 这些组合物包含由二肉豆蔻酰磷脂酰胆碱(“DMPC”)形成的脂质体; 二肉豆蔻酰磷脂酰甘油(“DMPG”)或二肉豆蔻酰基三甲基铵丙烷(“DMTAP”)或DMPC和DMTAP两者; 和至少一种甾醇; 和包含第一多肽序列的抗原多肽和第二多肽序列,所述第一多肽序列包含来自膜蛋白的跨膜序列,所述跨膜序列具有足以穿过脂质双层的残基数,至少9个连续残基 预测其形成疏水性评分为约0.7或更高的α螺旋。