公开(公告)号：US20190121941A1

公开(公告)日：2019-04-25

申请号：US16175742

申请日：2018-10-30

Applicant: GEN-PROBE INCORPORATED

Inventor： Tongjia Yin ,  Steve Brentano ,  Jennifer Bungo ,  Xianqun Wang ,  Michael Hadjisavas

IPC: G16B30/20

Abstract: The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.

公开(公告)号：US20140235461A1

公开(公告)日：2014-08-21

申请号：US14346954

申请日：2012-09-26

Applicant: Gen-Probe Incorporated

Inventor： Tongjia Yin ,  Steve Brentano ,  Jennifer Bungo ,  Xianqun Wang ,  Michael Hadjisavas

IPC: G06F19/22

CPC classification number: G06F19/22

Abstract: The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.

Abstract translation: 本发明提供了从核酸靶的多个原始测序读数确定共有序列的方法。 核酸靶包括已知序列的锚定区段和未知序列的相邻区段。 锚段提供了一种评估原始目标序列读取质量的方法。 原始目标排序读取会议或超过阈值被分配给接受的类。 相邻段的共有序列可以从接受的类中的原始目标测序读数确定。 连续轮询步骤确定相邻段的新生序列中的连续共有核碱基。 取决于与最近确定的共有核碱基和/或新生序列的对应关系，可以从接受的类别去除或重新引入原始靶序列读数。

公开(公告)号：US11862299B2

公开(公告)日：2024-01-02

申请号：US16175742

申请日：2018-10-30

Applicant: GEN-PROBE INCORPORATED

Inventor： Tongjia Yin ,  Steven T. Brentano ,  Jennifer Bungo ,  Xianqun Wang ,  Michael Hadjisavas

IPC: G16B30/00 ,  G16B30/10

CPC classification number: G16B30/00 ,  G16B30/10

Abstract: The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.

公开(公告)号：US10152569B2

公开(公告)日：2018-12-11

申请号：US14346954

申请日：2012-09-26

Applicant: Gen-Probe Incorporated

Inventor： Tongjia Yin ,  Steve Brentano ,  Jennifer Bungo ,  Xianqun Wang ,  Michael Hadjisavas

IPC: G06F19/22

Abstract: The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.