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公开(公告)号：US11274336B2
公开(公告)日：2022-03-15
申请号：US15912962
申请日：2018-03-06
发明人： Michael J. Gilly , Sangeetha Vijaysri Nair , James M. Carrick , Xianqun Wang , Susan K. Yamagata
IPC分类号： C12Q1/6844 , G16B40/00 , C12Q1/6851
摘要： Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The invention employs dual reference calibration curves for approximating a complete calibration curve from only a single adjustment calibrator amplified on the instrument that is to be calibrated.
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公开(公告)号：US20230392197A1
公开(公告)日：2023-12-07
申请号：US18236812
申请日：2023-08-22
IPC分类号： C12Q1/6844 , G16B40/00 , C12Q1/6851 , G16B40/10
CPC分类号： C12Q1/6844 , G16B40/00 , C12Q1/6851 , G16B40/10
摘要： Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The approach employs a single fixed data point in combination with a single adjustment calibrator amplified on the instrument that is to be calibrated for approximating a complete calibration curve.
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公开(公告)号：US09932628B2
公开(公告)日：2018-04-03
申请号：US13952338
申请日：2013-07-26
发明人： Michael J. Gilly , Sangeetha Vijaysri Nair , James M. Carrick , Xianqun Wang , Susan K. Yamagata
CPC分类号： C12Q1/6844 , C12Q1/6851 , G06F19/24 , C12Q2537/165
摘要： Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The invention employs dual reference calibration curves for approximating a complete calibration curve from only a single adjustment calibrator amplified on the instrument that is to be calibrated.
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公开(公告)号：US20140235461A1
公开(公告)日：2014-08-21
申请号：US14346954
申请日：2012-09-26
IPC分类号： G06F19/22
CPC分类号： G06F19/22
摘要： The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.
摘要翻译： 本发明提供了从核酸靶的多个原始测序读数确定共有序列的方法。 核酸靶包括已知序列的锚定区段和未知序列的相邻区段。 锚段提供了一种评估原始目标序列读取质量的方法。 原始目标排序读取会议或超过阈值被分配给接受的类。 相邻段的共有序列可以从接受的类中的原始目标测序读数确定。 连续轮询步骤确定相邻段的新生序列中的连续共有核碱基。 取决于与最近确定的共有核碱基和/或新生序列的对应关系，可以从接受的类别去除或重新引入原始靶序列读数。
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公开(公告)号：US20240318241A1
公开(公告)日：2024-09-26
申请号：US18733583
申请日：2024-06-04
IPC分类号： C12Q1/6865 , C12Q1/70 , G16B20/00 , G16B40/00
CPC分类号： C12Q1/6865 , G16B20/00 , G16B40/00 , C12Q1/703
摘要： Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.
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公开(公告)号：US11862299B2
公开(公告)日：2024-01-02
申请号：US16175742
申请日：2018-10-30
摘要： The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.
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公开(公告)号：US11120891B2
公开(公告)日：2021-09-14
申请号：US14414688
申请日：2013-07-11
发明人： Somalee Datta , Brett Bowman , Xianqun Wang , Mingjie Wang
IPC分类号： G16B40/00 , G16B20/00 , G16B20/20 , C12Q1/6809
摘要： Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.
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公开(公告)号：US10152569B2
公开(公告)日：2018-12-11
申请号：US14346954
申请日：2012-09-26
IPC分类号： G06F19/22
摘要： The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.
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公开(公告)号：US20150211079A1
公开(公告)日：2015-07-30
申请号：US14414688
申请日：2013-07-11
发明人： Somalee Datta , Brett Bowman , Xianqun Wang , Mingjie Wang
CPC分类号： C12Q1/707 , C12Q1/6809 , C12Q2600/156 , C12Q2600/16 , G16B20/00 , C12Q2535/122 , C12Q2537/165
摘要： Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants.
摘要翻译： 公开了用于检测靶核酸的少数基因型的方法。 所公开的方法通常包括以下步骤：（a）深入测序目标核酸的至少一部分; （b）使用（a）的深测序结果来检测靶核酸内的一个或多个核苷酸参考位置处的变体核碱基的存在; （c）使用步骤（b）中产生的变体检测结果来进行变异是否显着的统计分析; 和（d）使用在步骤（b）和（c）中产生的变体检测和变异性显着性结果来执行序列子集是否一起显示共同的一组重要变体的统计分析。
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公开(公告)号：US12037638B2
公开(公告)日：2024-07-16
申请号：US17783231
申请日：2020-12-09
IPC分类号： G06F30/20 , C12Q1/6865 , G16B20/00 , G16B40/00 , C12Q1/70
CPC分类号： C12Q1/6865 , G16B20/00 , G16B40/00 , C12Q1/703 , C12Q1/6865 , C12Q2537/165 , C12Q2545/114 , C12Q2561/113 , C12Q2565/625
摘要： Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.
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