公开(公告)号：US20040063203A1

公开(公告)日：2004-04-01

申请号：US10695605

申请日：2003-10-28

Applicant: GenVec, Inc.

Inventor： Douglas E. Brough ,  Jason G. D. Gall ,  Imre Kovesdi

IPC: C12N005/08 ,  C12N015/861

CPC classification number: C12N7/00 ,  C12N2710/10051 ,  C12N2710/10052 ,  C12N2710/10351 ,  C12N2710/10352

Abstract: The invention provides cells and methods of using the cells for the propagation of replication-deficient adenoviral vectors. The cells comprise at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.

Abstract translation: 本发明提供了细胞和使用细胞促进复制缺陷型腺病毒载体的方法。 所述细胞包含至少一种异源核酸序列，其在表达时产生至少一种非腺病毒基因产物，所述非腺病毒基因产物在腺病毒基因组的一个或多个区域的至少一个必需基因功能缺陷中互补互补，从而传播 复制缺陷型腺病毒载体，其包含存在于细胞中时所述一个或多个区域的至少一个必需基因功能缺陷的腺病毒基因组。

公开(公告)号：US20030017595A1

公开(公告)日：2003-01-23

申请号：US09911011

申请日：2001-07-23

Applicant: GenVec, Inc.

Inventor： Douglas E. Brough ,  Jason G. D. Gall ,  Imre Kovesdi

IPC: C12N015/861 ,  C12N007/01 ,  C12N005/08

CPC classification number: C12N7/00 ,  C12N2710/10051 ,  C12N2710/10052 ,  C12N2710/10351 ,  C12N2710/10352

Abstract: The invention provides cells and methods of using the cells for the propagation of replication-deficient adenoviral vectors. The cells comprise at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.

Abstract translation: 本发明提供了细胞和使用细胞促进复制缺陷型腺病毒载体的方法。 所述细胞包含至少一种异源核酸序列，其在表达时产生至少一种非腺病毒基因产物，所述非腺病毒基因产物在腺病毒基因组的一个或多个区域的至少一个必需基因功能缺陷中互补互补，从而传播 复制缺陷型腺病毒载体，其包含存在于细胞中时所述一个或多个区域的至少一个必需基因功能缺陷的腺病毒基因组。