公开(公告)号：US20020045160A1

公开(公告)日：2002-04-18

申请号：US09888681

申请日：2001-06-25

Applicant: GenVec, Inc.

Inventor： Miguel E. Carrion ,  Marilyn C. Menger

IPC: C12Q001/70 ,  C12N013/00 ,  G01N033/558 ,  G01N033/559 ,  G01N033/561

CPC classification number: G01N21/6486 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351

Abstract: The present invention provides methods of detecting and/or characterizing the viral vector particle content of a medium. A medium is provided and contacted with an excitation energy such that, if a viral vector particle is in the medium, an electron associated with the intrinsically fluorogenic portion of the viral vector particle will be raised to an excited energy state. The excited electron is permitted to emit radiation having an emission wavelength which is detected. The viral vector particle content of the medium then can be evaluated by comparing the detected emission wavelength with a standard signal. For example, the number of viral vector particles in a medium can be quantified by comparing the detected wavelength and its corresponding intensity to a standard signal. Similar methods for evaluating the adenoviral vector particle content of a medium and the intrinsically fluorogenic adenoviral structural protein content of a medium are provided.

Abstract translation: 本发明提供了检测和/或表征介质的病毒载体颗粒含量的方法。 提供介质并与激发能接触，使得如果病毒载体颗粒在介质中，与病毒载体颗粒的固有荧光部分相关的电子将被升高到激发能态。 被激发的电子被允许发射具有检测到的发射波长的辐射。 然后可以通过将检测到的发射波长与标准信号进行比较来评估介质的病毒载体颗粒含量。 例如，可以通过将检测到的波长及其对应的强度与标准信号进行比较来量化介质中病毒载体颗粒的数量。 提供了用于评估培养基的腺病毒载体颗粒含量和介质的固有荧光腺病毒结构蛋白含量的类似方法。