公开(公告)号：US20240246072A1

公开(公告)日：2024-07-25

申请号：US18425316

申请日：2024-01-29

申请人： GeneCapture, Inc.

发明人： Greg Thompson ,  Zachary McGee ,  Paula Millirons

IPC分类号： B01L3/00

CPC分类号： B01L3/502 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0436

摘要： Integrated cartridges for sample homogenization, nucleic acid fragmentation, and nucleic acid detection are disclosed herein. The integrated cartridges include a main housing having a sample well and a detection chamber and a sonication feature coupled to and extending outwardly from the main housing. The sonication feature includes a sonication chamber for receiving a sample fluid. A fluidic path directs the sample fluid from the sample well, to the sonication chamber, and to the detection chamber. Systems for use with the integrated cartridges can include sonotrodes with openings for positioning the sonication feature and/or temperature sensors for monitoring the temperature of the sonication feature. Methods include moving the sample fluid from the sample well to the sonication feature, transmitting ultrasonic energy into the sample fluid, moving the sample fluid from the sonication feature to the detection chamber, and performing a nucleic acid detection assay within the detection chamber.

公开(公告)号：US11918996B2

公开(公告)日：2024-03-05

申请号：US16848306

申请日：2020-04-14

申请人： GeneCapture, Inc.

发明人： Greg Thompson ,  Zachary McGee ,  Paula Millirons

IPC分类号： B01L3/00

CPC分类号： B01L3/502 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0436

摘要： Integrated cartridges for sample homogenization, nucleic acid fragmentation, and nucleic acid detection are disclosed herein. The integrated cartridges include a main housing having a sample well and a detection chamber and a sonication feature coupled to and extending outwardly from the main housing. The sonication feature includes a sonication chamber for receiving a sample fluid. A fluidic path directs the sample fluid from the sample well, to the sonication chamber, and to the detection chamber. Systems for use with the integrated cartridges can include sonotrodes with openings for positioning the sonication feature and/or temperature sensors for monitoring the temperature of the sonication feature. Methods include moving the sample fluid from the sample well to the sonication feature, transmitting ultrasonic energy into the sample fluid, moving the sample fluid from the sonication feature to the detection chamber, and performing a nucleic acid detection assay within the detection chamber.