公开(公告)号：US20240361211A1

公开(公告)日：2024-10-31

申请号：US18738633

申请日：2024-06-10

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Jeffrey R. Jasperse ,  Peter Paulicka ,  Paul Benson

IPC分类号： G01N1/28 ,  B01L3/00 ,  G01N29/036 ,  G01N29/34

CPC分类号： G01N1/286 ,  B01L3/502715 ,  G01N29/036 ,  G01N29/348 ,  B01L2300/0627 ,  B01L2300/0861 ,  B01L2300/12 ,  B01L2400/0436

摘要： A lysis device including a sample vessel, at least one piezo element, and a controller is disclosed. The sample vessel has a microchannel formed therein. The sample vessel has at least one port extending through a surface to the microchannel. The piezo element is attached to the surface of the sample vessel. The controller has logic to cause the controller to emit a first signal including a series of frequencies to the at least one piezo element to cause the at least one piezo element to generate ultrasonic acoustic standing waves in the sample vessel, to receive a second signal indicative of measured vibration signals from the sample vessel detected by the at least one piezo element, and to determine a resonant frequency of the sample vessel using the measured vibration signals.

公开(公告)号：US20240345102A1

公开(公告)日：2024-10-17

申请号：US18682419

申请日：2022-08-05

申请人： Siemens Healthcare Diagnostics

发明人： Tivadar Mach ,  Peter Paulicka ,  Ramona Seliger ,  Frederick Stelling

IPC分类号： G01N33/72 ,  B01L3/00 ,  G01N33/80 ,  G01N33/86 ,  G01N33/92

CPC分类号： G01N33/72 ,  B01L3/502761 ,  G01N33/80 ,  G01N33/86 ,  G01N33/92 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2400/0436

摘要： The disclosure relates to a device and method for analyzing a blood sample, particularly including pre-analytical (qualification), analytical and post-analytical (verification) testing. More particularly the disclosure relates in certain embodiments to a device configured for collecting, imaging and lysing blood cells in a sample vessel by means of ultrasonic acoustic waves, generated in the vessel by an acoustic transducer driven at one particular excitation frequency or more particular excitation frequencies, or one range of frequency or ranges of frequencies. In some non-limiting embodiments, the ultrasonic acoustic waves are generated by a single acoustic transducer. Further on, according to certain embodiments, the device and workflow are configured for three analytical steps in single device, comprising pre-analytical (qualification), analytical and post-analytical (verification) testing.

公开(公告)号：US20240316550A1

公开(公告)日：2024-09-26

申请号：US18574549

申请日：2022-06-30

申请人： LUMICKS CA Holding B.V.

发明人： Douwe Kamsma ,  Felix Oswald ,  Amanda Caroline Franklin

IPC分类号： B01L3/00 ,  G01H1/00

CPC分类号： B01L3/50273 ,  G01H1/00 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2400/0436

摘要： A method is disclosed for characterizing an acoustic-based particle manipulation device comprising a sample holder having a holding space containing a fluid medium. The holding space comprises a wall surface portion. The method comprises providing a driving signal to the sample holder for generating an acoustic wave in the holding space that drives a particle at the wall surface portion, away from the wall surface portion. The method further comprises measuring vibrations of at least part of a surface of the sample holder caused by said driving signal. The method also comprises, based on the measured vibrations of said at least part of the surface, determining, for each position out of a plurality of positions at the wall surface portion and in the holding space, a value for an acoustic force that is caused by said driving signal and that acts in a direction away from the wall surface portion.

公开(公告)号：US20240278241A1

公开(公告)日：2024-08-22

申请号：US18637954

申请日：2024-04-17

申请人： CytoVale Inc.

发明人： Henry Tat Kwong Tse ,  Ajay M. Shah ,  Lionel Guillou ,  Roya Sheybani ,  Christopher Dahlberg ,  Anne E. Jensen

IPC分类号： B01L3/00 ,  G06N3/02 ,  G06T7/00

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G06N3/02 ,  G06T7/0012 ,  B01L2200/0631 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/0694 ,  B01L2200/12 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0436 ,  G06T2207/30024 ,  G06T2207/30104

摘要： A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.

公开(公告)号：US12059679B2

公开(公告)日：2024-08-13

申请号：US17144575

申请日：2021-01-08

申请人： 10X Genomics, Inc.

发明人： Alireza Salmanzadeh ,  Rajiv Bharadwaj

IPC分类号： G01N29/22 ,  B01L3/00

CPC分类号： B01L3/502761 ,  G01N29/222 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0436 ,  B01L2400/0439

摘要： Devices, systems, and their methods of use, for sorting droplets or particles are provided. A source of acoustic energy can be employed to sort droplets or particles of a desired and predictable property.

公开(公告)号：US20240246072A1

公开(公告)日：2024-07-25

申请号：US18425316

申请日：2024-01-29

申请人： GeneCapture, Inc.

发明人： Greg Thompson ,  Zachary McGee ,  Paula Millirons

IPC分类号： B01L3/00

CPC分类号： B01L3/502 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0436

摘要： Integrated cartridges for sample homogenization, nucleic acid fragmentation, and nucleic acid detection are disclosed herein. The integrated cartridges include a main housing having a sample well and a detection chamber and a sonication feature coupled to and extending outwardly from the main housing. The sonication feature includes a sonication chamber for receiving a sample fluid. A fluidic path directs the sample fluid from the sample well, to the sonication chamber, and to the detection chamber. Systems for use with the integrated cartridges can include sonotrodes with openings for positioning the sonication feature and/or temperature sensors for monitoring the temperature of the sonication feature. Methods include moving the sample fluid from the sample well to the sonication feature, transmitting ultrasonic energy into the sample fluid, moving the sample fluid from the sonication feature to the detection chamber, and performing a nucleic acid detection assay within the detection chamber.

公开(公告)号：US20240198338A1

公开(公告)日：2024-06-20

申请号：US18595920

申请日：2024-03-05

申请人： The General Hospital Corporation

发明人： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC分类号： B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/01 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N15/149

CPC分类号： B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N15/01 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N15/149 ,  G01N2015/1493

摘要： A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：US11944971B2

公开(公告)日：2024-04-02

申请号：US17245770

申请日：2021-04-30

申请人： The General Hospital Corporation

发明人： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC分类号： B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14

CPC分类号： B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

摘要： A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：US11918996B2

公开(公告)日：2024-03-05

申请号：US16848306

申请日：2020-04-14

申请人： GeneCapture, Inc.

发明人： Greg Thompson ,  Zachary McGee ,  Paula Millirons

IPC分类号： B01L3/00

CPC分类号： B01L3/502 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0436

摘要： Integrated cartridges for sample homogenization, nucleic acid fragmentation, and nucleic acid detection are disclosed herein. The integrated cartridges include a main housing having a sample well and a detection chamber and a sonication feature coupled to and extending outwardly from the main housing. The sonication feature includes a sonication chamber for receiving a sample fluid. A fluidic path directs the sample fluid from the sample well, to the sonication chamber, and to the detection chamber. Systems for use with the integrated cartridges can include sonotrodes with openings for positioning the sonication feature and/or temperature sensors for monitoring the temperature of the sonication feature. Methods include moving the sample fluid from the sample well to the sonication feature, transmitting ultrasonic energy into the sample fluid, moving the sample fluid from the sonication feature to the detection chamber, and performing a nucleic acid detection assay within the detection chamber.

公开(公告)号：US11890870B2

公开(公告)日：2024-02-06

申请号：US17289552

申请日：2019-10-29

申请人： Labcyte Inc.

发明人： Sammy S. Datwani ,  Carson Riche ,  Marsha N. Blauwkamp ,  Justin Blonigan

IPC分类号： B41J2/045 ,  B01L3/02 ,  B01J19/00 ,  B01J19/26

CPC分类号： B41J2/04575 ,  B01J19/0046 ,  B01J19/26 ,  B01L3/0268 ,  B41J2/04516 ,  B41J2/04588 ,  B01J2219/00362 ,  B01L2200/0647 ,  B01L2200/148 ,  B01L2400/0436 ,  B01L2400/0439

摘要： Methods of ejecting droplets containing a non-Newtonian fluid by an acoustic droplet ejector can include applying a tone burst of focused acoustic energy to a fluid reservoir containing a non-Newtonian fluid at sufficient amplitude to effect droplet ejection according to a tone burst pattern. The tone burst pattern may include three discrete tone burst segments, the first tone burst segment having greater duration than the second and third segments, and third segment having greater duration than the second segment. The exact durations and amplitudes of the tone burst segments can be tuned to influence the ejection properties.