-
公开(公告)号：US20220119985A1
公开(公告)日：2022-04-21
申请号：US17562950
申请日：2021-12-27
Applicant: GENENTECH, INC.
Inventor： Shahram MISAGHI , Bradley R. SNEDECOR
Abstract: The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.
-
公开(公告)号：US20250137027A1
公开(公告)日：2025-05-01
申请号：US18990600
申请日：2024-12-20
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
-
公开(公告)号：US20180291411A1
公开(公告)日：2018-10-11
申请号：US15908526
申请日：2018-02-28
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
-
Patent Agency Ranking
公开(公告)号：US20240158825A1
公开(公告)日：2024-05-16
申请号：US18419368
申请日：2024-01-22
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
-
公开(公告)号：US20150225760A1
公开(公告)日：2015-08-13
申请号：US14497964
申请日：2014-09-26
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L E ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates methods of producing a recombinant protein comprising fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. Furthermore, method of producing a recombinant protein comprising fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, wherein the recombinant protein yield is increased by about 20% or greater is contemplated.
Abstract translation: 本发明涉及生产包含发酵原核宿主细胞的重组蛋白的方法，其中所述原核宿主细胞已用编码所述重组蛋白的核酸转化，在dO2水平大于0％的条件下收获所述重组蛋白，纯化所述 重组蛋白转化成过滤体积，其中所述过滤的体积不含可检测的DHNA重组蛋白质加合物，如通过IEC测定在310nm测量的。 此外，制备包含发酵menE基因缺失的原核宿主细胞的重组蛋白质的方法，其中所述原核宿主细胞已经用编码所述重组蛋白的核酸转化，收获所述重组蛋白质，将所述重组蛋白质纯化成过滤体积，其中 所述过滤体积不包含可检测的DHNA重组蛋白质加合物，如通过在310nm的IEC测定法测定的，其中重组蛋白质产量提高约20％或更高。
-
公开(公告)号：US20230069966A1
公开(公告)日：2023-03-09
申请号：US17864255
申请日：2022-07-13
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
-
公开(公告)号：US20210171997A1
公开(公告)日：2021-06-10
申请号：US17067059
申请日：2020-10-09
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
-
公开(公告)号：US20160130624A1
公开(公告)日：2016-05-12
申请号：US14734848
申请日：2015-06-09
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
Abstract translation: 本发明涉及生产重组蛋白的方法，其包括（a）发酵原核宿主细胞，其中所述原核宿主细胞已经用编码所述重组蛋白的核酸转化，和（b）在dO2水平的条件下收获所述重组蛋白 大于0％，和（c）将所述重组蛋白纯化至过滤的体积，其中所述过滤的体积不含可检测的DHNA重组蛋白加合物，如通过IEC测定在310nm测量的。 本发明还涉及一种生产重组蛋白的方法，其包括（a）发酵menE基因缺失的原核宿主细胞，其中所述原核宿主细胞已用编码所述重组蛋白的核酸转化，（b）收获所述重组蛋白; 和（c）将所述重组蛋白纯化至过滤体积，其中所述过滤的体积不含可检测的DHNA重组蛋白质加合物，如通过IEC测定在310nm测量的，并且其中重组蛋白质产量增加约20％或 更大
-
-
-
-
-
-
-
Search Results
8
records
(took 0.012 seconds)
