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公开(公告)号：US20250137027A1
公开(公告)日：2025-05-01
申请号：US18990600
申请日：2024-12-20
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20180291411A1
公开(公告)日：2018-10-11
申请号：US15908526
申请日：2018-02-28
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20220169675A1
公开(公告)日：2022-06-02
申请号：US17328408
申请日：2021-05-24
Applicant: Genentech, Inc. , Hoffmann-La Roche Inc.
Inventor： Paul MCDONALD , Richard ST. JOHN , Marc Wong , Roberto FALKENSTEIN , Wolfgang KOEHNLEIN , Klaus SCHWENDNER , Bernhard SPENSBERGER , Michael WIEDMANN , Frank ZETTL , Annika KLEINJANS , Carina KOPP , Benjamin TRAN , Ryan ERICKSON
Abstract: The current invention reports a method for purifying an antibody by reducing the content of a host cell protein. The method employs a wash step with a low conductivity aqueous solution in an affinity chromatography.
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公开(公告)号：US20180186832A1
公开(公告)日：2018-07-05
申请号：US15900461
申请日：2018-02-20
Applicant: Genentech, Inc. , Hoffmann-La Roche Inc.
Inventor： Paul MCDONALD , Richard ST. JOHN , Marc WONG , Roberto FALKENSTEIN , Wolfgang KOEHNLEIN , Klaus SCHWENDNER , Bernhard SPENSBERGER , Michael WIEDMANN , Frank ZETTL , Annika KLEINJANS , Carina KOPP , Benjamin TRAN , Ryan ERICKSON
CPC classification number: C07K1/22 , C07K16/18 , C07K16/22 , C07K16/2854 , C07K16/32 , C07K16/36 , C07K2317/14 , C07K2317/31
Abstract: The current invention reports a method for purifying an antibody by reducing the content of a host cell protein. The method employs a wash step with a low conductivity aqueous solution in an affinity chromatography.
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Patent Agency Ranking
公开(公告)号：US20240158825A1
公开(公告)日：2024-05-16
申请号：US18419368
申请日：2024-01-22
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20150225760A1
公开(公告)日：2015-08-13
申请号：US14497964
申请日：2014-09-26
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L E ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates methods of producing a recombinant protein comprising fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. Furthermore, method of producing a recombinant protein comprising fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, wherein the recombinant protein yield is increased by about 20% or greater is contemplated.
Abstract translation: 本发明涉及生产包含发酵原核宿主细胞的重组蛋白的方法，其中所述原核宿主细胞已用编码所述重组蛋白的核酸转化，在dO2水平大于0％的条件下收获所述重组蛋白，纯化所述 重组蛋白转化成过滤体积，其中所述过滤的体积不含可检测的DHNA重组蛋白质加合物，如通过IEC测定在310nm测量的。 此外，制备包含发酵menE基因缺失的原核宿主细胞的重组蛋白质的方法，其中所述原核宿主细胞已经用编码所述重组蛋白的核酸转化，收获所述重组蛋白质，将所述重组蛋白质纯化成过滤体积，其中 所述过滤体积不包含可检测的DHNA重组蛋白质加合物，如通过在310nm的IEC测定法测定的，其中重组蛋白质产量提高约20％或更高。
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公开(公告)号：US20230069966A1
公开(公告)日：2023-03-09
申请号：US17864255
申请日：2022-07-13
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20210171997A1
公开(公告)日：2021-06-10
申请号：US17067059
申请日：2020-10-09
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20160130624A1
公开(公告)日：2016-05-12
申请号：US14734848
申请日：2015-06-09
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
Abstract translation: 本发明涉及生产重组蛋白的方法，其包括（a）发酵原核宿主细胞，其中所述原核宿主细胞已经用编码所述重组蛋白的核酸转化，和（b）在dO2水平的条件下收获所述重组蛋白 大于0％，和（c）将所述重组蛋白纯化至过滤的体积，其中所述过滤的体积不含可检测的DHNA重组蛋白加合物，如通过IEC测定在310nm测量的。 本发明还涉及一种生产重组蛋白的方法，其包括（a）发酵menE基因缺失的原核宿主细胞，其中所述原核宿主细胞已用编码所述重组蛋白的核酸转化，（b）收获所述重组蛋白; 和（c）将所述重组蛋白纯化至过滤体积，其中所述过滤的体积不含可检测的DHNA重组蛋白质加合物，如通过IEC测定在310nm测量的，并且其中重组蛋白质产量增加约20％或 更大
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