公开(公告)号：US10073098B2

公开(公告)日：2018-09-11

申请号：US15061943

申请日：2016-03-04

Applicant: Genentech, Inc.

Inventor： Marc Wong ,  Liliana T. Yee ,  Amy Lim ,  Chris B. Fong

IPC: G01N33/53 ,  G01N33/573 ,  C12N9/02 ,  C12N9/90 ,  C07K1/22 ,  C07K16/40 ,  A61K38/00

Abstract: The present invention provides methods for producing disulfide oxidoreductase A (DsbA) and disulfide oxidoreductase C (DsbC) polypeptides at very high levels of purity. Also provided are ultrapure DsbA and DsbC and methods of using same, e.g., for use in immunoassays to show removal of DsbA and DsbC from biologics produced in bacteria.

公开(公告)号：US10690671B2

公开(公告)日：2020-06-23

申请号：US16044360

申请日：2018-07-24

Applicant: Genentech, Inc.

Inventor： Marc Wong ,  Liliana T. Yee ,  Amy Lim ,  Chris B. Fong

IPC: G01N33/53 ,  G01N33/573 ,  C12N9/02 ,  C12N9/90 ,  C07K1/22 ,  C07K16/40 ,  A61K38/00

Abstract: The present invention provides methods for producing disulfide oxidoreductase A (DsbA) and disulfide oxidoreductase C (DsbC) polypeptides at very high levels of purity. Also provided are ultrapure DsbA and DsbC and methods of using same, e.g., for use in immunoassays to show removal of DsbA and DsbC from biologics produced in bacteria.

公开(公告)号：US20190056399A1

公开(公告)日：2019-02-21

申请号：US16044360

申请日：2018-07-24

Applicant: Genentech, Inc.

Inventor： Marc Wong ,  Liliana T. Yee ,  Amy Lim ,  Chris B. Fong

IPC: G01N33/573 ,  C12N9/90 ,  C07K1/22 ,  C07K16/40 ,  C12N9/02 ,  A61K38/00

Abstract: The present invention provides methods for producing disulfide oxidoreductase A (DsbA) and disulfide oxidoreductase C (DsbC) polypeptides at very high levels of purity. Also provided are ultrapure DsbA and DsbC and methods of using same, e.g., for use in immunoassays to show removal of DsbA and DsbC from biologics produced in bacteria.