公开(公告)号：US20220127649A1

公开(公告)日：2022-04-28

申请号：US17427019

申请日：2020-01-30

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Jonathan Moore ,  Jingyi Li ,  Anna Lechner

IPC: C12P7/42 ,  C12N1/20 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22

Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing synthesis of olivetolic acid, olivetolic acid derivatives and/or cannabinoids.

公开(公告)号：US20230374473A1

公开(公告)日：2023-11-23

申请号：US18316993

申请日：2023-05-12

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Kevin G. Hoff ,  Anna Lechner ,  Harish Nagarajan

IPC: C12N9/10 ,  C12P7/22 ,  C12P7/42

CPC classification number: C12N9/1085 ,  C12P7/22 ,  C12P7/42

Abstract: Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US11685908B2

公开(公告)日：2023-06-27

申请号：US16978629

申请日：2019-03-08

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Kevin G. Hoff ,  Anna Lechner ,  Harish Nagarajan

IPC: C12N9/10 ,  C12P7/22 ,  C12P7/42

CPC classification number: C12N9/1085 ,  C12P7/22 ,  C12P7/42

Abstract: Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US20210254030A1

公开(公告)日：2021-08-19

申请号：US16978629

申请日：2019-03-08

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Kevin G. Hoff ,  Anna Lechner ,  Harish Nagarajan

IPC: C12N9/10 ,  C12P7/42 ,  C12P7/22

Abstract: Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.