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公开(公告)号：US5543302A
公开(公告)日：1996-08-06
申请号：US159211
申请日：1993-11-30
CPC分类号： C12N9/54 , C11D3/38618 , C12N9/50
摘要： Proteases are susceptible to autolytic degradation. The method of the invention provides a method to identify susceptible autolysis sites which are cleaved to produce a few discrete fragments. Once identified, amino acid(s) at or in the vicinity of the cleavage site may be chemically modified or the DNA sequence capable of encoding the amino acid present at the site may be changed or deleted by site directed mutagenesis to produce an autolysis stable protease. The invention is particularly applicable to alkaline proteases which are produced in large quantities and used as detergent additives. Stable alkaline proteases could then be used in liquid detergent formulations.
摘要翻译： 蛋白酶易于自溶降解。 本发明的方法提供了一种识别敏感的自溶位点的方法，其被切割以产生几个离散的片段。 一旦鉴定，可以化学修饰在切割位点处或其附近的氨基酸，或者可以通过定点诱变来改变或删除存在于位点的氨基酸的DNA序列以产生自溶稳定蛋白酶 。 本发明特别适用于大量生产并用作洗涤剂添加剂的碱性蛋白酶。 稳定的碱性蛋白酶可用于液体洗涤剂配方。
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公开(公告)号：US4666843A
公开(公告)日：1987-05-19
申请号：US787998
申请日：1985-10-16
IPC分类号： C12N9/64
CPC分类号： C12N9/6481 , Y10S435/815
摘要： Disclosed is a method for the purification of calf rennet which involves contacting the rennet with an affinity ligand, e.g. Cibacron Blue, to cause separation of the chymosin and pepsin contained in the rennet.
摘要翻译： 公开了一种纯化小牛凝乳酶的方法，其包括使凝乳酶与亲和配体接触，例如， Cibacron Blue，以使凝乳酶中包含的凝乳酶和胃蛋白酶分离。
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公开(公告)号：US4743551A
公开(公告)日：1988-05-10
申请号：US668404
申请日：1984-11-05
CPC分类号： C12N9/58 , Y10S435/815
摘要： A method for the purification of a culture filtrate resulting from the fermentation of an organism of the species Mucor miehei involves the selective adsorption of the microbial rennet present in the culture filtrate by a blue dye affinity ligand with subsequent elution of the adsorbed microbial rennet to provide it in a purified form.
摘要翻译： 用于纯化由Mucor miehei的生物的发酵产生的培养滤液的方法涉及通过蓝色染料亲和配体选择性吸附存在于培养滤液中的微生物凝乳酶，随后吸附的微生物凝乳酶洗脱以提供 它以纯化的形式。
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