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公开(公告)号：US06994963B1
公开(公告)日：2006-02-07
申请号：US09613535
申请日：2000-07-10
CPC分类号： C12N15/1027 , C12P19/34
摘要： This invention pertains to a method for generating a pool of nucleic acid fragments useful for in vitro recombination and the creation of novel DNA sequences that encode desirable proteins or enzymes. The invention provides a defined mixture of nucleic acids and methods for use in the synthesis, mutagenesis, and recombination of nucleic acids. Nucleic acids may be synthesized by creating a nucleic acid extension ladder, annealing the extension ladder to template nucleic acids, and further extending the ladder of nucleic acids. The invention also relates to methods for performing repeated cycles of synthesis for the purpose of mutagenesis or recombination, methods for producing mutant peptides and proteins from the mutagenized or recombined nucleic acids, and methods for selecting a peptide, polypeptide or protein having altered biological activities.
摘要翻译： 本发明涉及用于产生用于体外重组的核酸片段库的集合，以及产生编码所需蛋白质或酶的新型DNA序列的方法。 本发明提供用于核酸的合成，诱变和重组的核酸和方法的限定混合物。 可以通过产生核酸延伸梯，将延伸梯退火至模板核酸并进一步延长核酸梯级来合成核酸。 本发明还涉及用于进行突变或重组的重复合成循环的方法，用于从诱变或重组的核酸产生突变型肽和蛋白质的方法，以及用于选择具有改变的生物活性的肽，多肽或蛋白质的方法。
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公开(公告)号：US07595179B2
公开(公告)日：2009-09-29
申请号：US10827498
申请日：2004-04-19
CPC分类号： C12N9/1276 , C12Y207/07049
摘要： The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.
摘要翻译： 本发明涉及编码具有DNA聚合酶活性和显着降低的RNA酶H活性的多反应逆转录酶的基因，含有本发明转化的基因和宿主细胞的载体。 本发明还包括使用本发明的逆转录酶产生过度逆转录酶，从mRNA产生cDNA的方法，使用过度反转录酶制备的试剂盒和测定模板。
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公开(公告)号：US08426126B2
公开(公告)日：2013-04-23
申请号：US10955974
申请日：2004-09-30
申请人： Gary J. Latham , Xingwang Fang , Richard C. Conrad , Jon A. Kemppainen , Robert A. Setterquist , Brittan L. Pasloske
发明人： Gary J. Latham , Xingwang Fang , Richard C. Conrad , Jon A. Kemppainen , Robert A. Setterquist , Brittan L. Pasloske
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/1013
摘要： The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.
摘要翻译： 本发明涉及通过可逆地将多核苷酸结合到固体表面如磁性微粒从含有多核苷酸的溶液中分离多核苷酸如DNA，RNA和PNA的方法。
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公开(公告)号：US08361754B2
公开(公告)日：2013-01-29
申请号：US12568364
申请日：2009-09-28
IPC分类号： C12P19/34
CPC分类号： C12N9/1276 , C12Y207/07049
摘要： The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.
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公开(公告)号：US20100203597A1
公开(公告)日：2010-08-12
申请号：US12568364
申请日：2009-09-28
CPC分类号： C12N9/1276 , C12Y207/07049
摘要： The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.
摘要翻译： 本发明涉及编码具有DNA聚合酶活性和显着降低的RNA酶H活性的多反应逆转录酶的基因，含有本发明转化的基因和宿主细胞的载体。 本发明还包括使用本发明的逆转录酶产生过度逆转录酶，从mRNA产生cDNA的方法，使用过度反转录酶制备的试剂盒和测定模板。
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