公开(公告)号：US20090053724A1

公开(公告)日：2009-02-26

申请号：US12215455

申请日：2008-06-27

申请人： George Thomas Roth ,  John Richard Nobile ,  Maithreyan Srinivasan ,  Zhoutao Chen ,  James Matthew Nealis ,  Xavier Victor Gomes

发明人： George Thomas Roth ,  John Richard Nobile ,  Maithreyan Srinivasan ,  Zhoutao Chen ,  James Matthew Nealis ,  Xavier Victor Gomes

IPC分类号： C12Q1/68 ,  C12Q1/00 ,  C12M1/36 ,  C12M1/34 ,  C12Q1/34

CPC分类号： C12Q1/6869 ,  C12Q2565/301

摘要： An embodiment of a method for adaptive reagent control is described that comprising a) introducing a first concentration of an enzyme reagent into a reaction environment with a reaction substrate, where the enzyme reagent and reaction substrate are constituent parts of a sequencing process; b) measuring a level of activity of the first concentration of the enzyme reagent in the reaction environment, where the level of activity comprises a measurable product of a reaction between the enzyme reagent and the reaction substrate; c) identifying an optimal concentration using the measured level of activity of the first concentration; and d) performing the sequencing process in the reaction environment using the optimal concentration of the enzyme reagent, where the sequencing process comprises an iterative series of sequencing reactions.

摘要翻译： 描述了一种用于自适应试剂控制的方法的实施方案，其包括：a）将酶试剂的第一浓度与反应底物引入反应环境，其中酶试剂和反应底物是测序过程的组成部分; b）测量反应环境中酶试剂的第一浓度的活性水平，其中活性水平包括酶试剂和反应底物之间的反应的可测量的产物; c）使用所测量的第一浓度的活性水平鉴定最佳浓度; 和d）使用酶试剂的最佳浓度在反应环境中进行测序过程，其中测序过程包括迭代的一系列测序反应。