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1.发明申请公开(公告)号：US20090053724A1
公开(公告)日：2009-02-26
申请号：US12215455
申请日：2008-06-27
申请人： George Thomas Roth , John Richard Nobile , Maithreyan Srinivasan , Zhoutao Chen , James Matthew Nealis , Xavier Victor Gomes
发明人： George Thomas Roth , John Richard Nobile , Maithreyan Srinivasan , Zhoutao Chen , James Matthew Nealis , Xavier Victor Gomes
CPC分类号： C12Q1/6869 , C12Q2565/301
摘要： An embodiment of a method for adaptive reagent control is described that comprising a) introducing a first concentration of an enzyme reagent into a reaction environment with a reaction substrate, where the enzyme reagent and reaction substrate are constituent parts of a sequencing process; b) measuring a level of activity of the first concentration of the enzyme reagent in the reaction environment, where the level of activity comprises a measurable product of a reaction between the enzyme reagent and the reaction substrate; c) identifying an optimal concentration using the measured level of activity of the first concentration; and d) performing the sequencing process in the reaction environment using the optimal concentration of the enzyme reagent, where the sequencing process comprises an iterative series of sequencing reactions.
摘要翻译： 描述了一种用于自适应试剂控制的方法的实施方案，其包括：a）将酶试剂的第一浓度与反应底物引入反应环境，其中酶试剂和反应底物是测序过程的组成部分; b）测量反应环境中酶试剂的第一浓度的活性水平，其中活性水平包括酶试剂和反应底物之间的反应的可测量的产物; c）使用所测量的第一浓度的活性水平鉴定最佳浓度; 和d）使用酶试剂的最佳浓度在反应环境中进行测序过程，其中测序过程包括迭代的一系列测序反应。
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公开(公告)号：US11333440B2
公开(公告)日：2022-05-17
申请号：US17113811
申请日：2020-12-07
申请人： John Richard Nobile
发明人： John Richard Nobile
摘要： A first conduit having a cylindrical upper entrance region with the axis of the cylindrical region oriented vertically, which accepts incoming downwardly flowing hot drain water, a central region of said first conduit below the said entrance region having a conical shape of increasing diameter, reaching a maximum diameter 2-7 times larger than the upper region, the shape then transitioning to a decreasing diameter area of a conical shape, the shape transitioning to a cylindrical lower region with a diameter similar to the upper region diameter; a second conduit for the flow of the shower cold water supply, with a diameter 10-40 times smaller than the maximum diameter of the drain water conduit, and a length 10-40 times longer than the vertical length of the drain water conduit, the second conduit tightly wrapped around the first conduit and in close thermal contact with the first conduit.
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公开(公告)号：US20210172683A1
公开(公告)日：2021-06-10
申请号：US17113811
申请日：2020-12-07
申请人： John Richard Nobile
发明人： John Richard Nobile
摘要： A first conduit having a cylindrical upper entrance region with the axis of the cylindrical region oriented vertically, which accepts incoming downwardly flowing hot drain water, a central region of said first conduit below the said entrance region having a conical shape of increasing diameter, reaching a maximum diameter 2-7 times larger than the upper region, the shape then transitioning to a decreasing diameter area of a conical shape, the shape transitioning to a cylindrical lower region with a diameter similar to the upper region diameter; a second conduit for the flow of the shower cold water supply, with a diameter 10-40 times smaller than the maximum diameter of the drain water conduit, and a length 10-40 times longer than the vertical length of the drain water conduit, the second conduit tightly wrapped around the first conduit and in close thermal contact with the first conduit.
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公开(公告)号：US20230094765A1
公开(公告)日：2023-03-30
申请号：US17935423
申请日：2022-09-26
申请人： John Richard Nobile
发明人： John Richard Nobile
IPC分类号： B01L3/00
摘要： An automatic nucleic acid extraction cartridge and an automatic nucleic acid extraction system including the same are described herein. The cartridge having a housing that includes a sample port, a cell processing chamber, a wash fluid chamber, a filter assembly comprising a filter member, and a diverter valve having a first and a second reversibly sealable output, wherein each of the sample port and the cell processing chamber, the cell processing chamber and the filter assembly, and the wash fluid chamber and the filter assembly are in one-way fluid communication, and the filter assembly is in fluid communication with the diverter valve and (i) a waste conduit when the diverter valve is biased to the first reversibly sealable output and (ii) a pathogen nucleic acid conduit when the diverter valve is biased to the second reversibly sealable output. The present disclosure further describes methods of using the same.
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