公开(公告)号：US06150081A

公开(公告)日：2000-11-21

申请号：US219849

申请日：1998-12-23

申请人： George Valentino Van Heerde ,  Alexis Cornelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richele Deodata Wind ,  Tanja Jacoba Van Den Bosch

发明人： George Valentino Van Heerde ,  Alexis Cornelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richele Deodata Wind ,  Tanja Jacoba Van Den Bosch

IPC分类号： C12N15/09 ,  A61K38/17 ,  C07K5/04 ,  C07K14/78 ,  C12N1/19 ,  C12N1/21 ,  C12R1/66 ,  C12R1/78 ,  C12R1/84 ,  C12R1/885 ,  G03C1/005 ,  G03C1/015 ,  G03C1/035 ,  G03C1/047

CPC分类号： C07K14/78 ,  G03C1/0051 ,  G03C1/047

摘要： A tabular silver halide emulsion wherein the tabular grains account for more than 75% of the total grain projected area said emulsion comprising silver halide grains nucleated in the presence of nucleation peptizer and thereafter grown in the presence of growth peptizer, wherein at least one of the peptizers is substantially pure collagen like material prepared by genetic engineering, said peptizer having an amino acid sequence comprising more than 4 different amino acids. A process of preparing the AgX emulsion. A process of producing recombinant collagen like polypeptide comprising expression of a collagen like polypeptide encoding nucleic acid sequence by a microorganism to a degree exceeding 0.95 grammes/liter, said recombinant collagen being free of helix structure and preferably the expression occurring in a microorganism other than E. coli or Saccharomyces cerevisiae.

摘要翻译： 一种片状卤化银乳剂，其中片状颗粒占总颗粒投影面积的75％以上，所述乳状液包含在成核胶溶剂存在下成核的卤化银颗粒，然后在生长胶溶剂存在下生长，其中至少一种 胶溶剂是通过基因工程制备的基本上纯的胶原蛋白，所述胶溶剂具有包含多于4种不同氨基酸的氨基酸序列。 制备AgX乳液的方法。 一种生产重组胶原蛋白样多肽的方法，包括将微生物编码核酸序列的胶原蛋白多肽表达至超过0.95克/升的程度，所述重组胶原蛋白不含螺旋结构，优选在除E以外的微生物中发生的表达 大肠杆菌或酿酒酵母（Saccharomyces cerevisiae）。