公开(公告)号：US10821174B2

公开(公告)日：2020-11-03

申请号：US16088255

申请日：2017-03-16

申请人： Hemu Wang

发明人： Hemu Wang ,  Changhua Wang ,  Jun Yang

IPC分类号： A61K39/29 ,  C12N9/88 ,  A61K39/12 ,  A61P31/20 ,  A61K39/39 ,  C07K14/02 ,  C12R1/78 ,  A61K39/00

摘要： Provided is a recombinant Hansenula polymorpha-based high dosage hepatitis B vaccine, an HBsAg pure stock solution yield of a recombinant Hansenula polymorpha fermentation broth used for producing the hepatitis B vaccine being 300 mg/L-400 mg/L.

公开(公告)号：US5527693A

公开(公告)日：1996-06-18

申请号：US391480

申请日：1995-02-21

申请人： Rosanna Cardillo ,  Claudio Fuganti ,  Massimo Barbeni ,  Gianna Allegrone

发明人： Rosanna Cardillo ,  Claudio Fuganti ,  Massimo Barbeni ,  Gianna Allegrone

IPC分类号： C07D309/30 ,  A23L27/20 ,  A23L27/24 ,  C12P17/06 ,  C12P17/08 ,  C12R1/645 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85 ,  C12P1/02 ,  C12P7/42

CPC分类号： C12P17/06 ,  A23L27/2052 ,  A23L27/24

摘要： The production of natural delta-decanolide and/or delta-dodecanolide or mixtures thereof, by means of the biohydrogenation of a substrate containing the corresponding unsaturated lactones, delta-decen-2-olide and delta-dodecen-2-olide, respectively, and mixtures thereof, is performed by means of the use of a microorganism chosen from the group consisting of Saccharomyces delbrueckii, Pichia ohmeri, Hansenula anomala, Pichia stipitis, Debaromyces hansenii, Zymomonas mobilis, Zygosaccharomyces rouxii, Schawnniomyces occidentalis, Sarcina lutea and Geotrichum candidum.

摘要翻译： 通过分别含有相应的不饱和内酯，δ-癸烯-2-醇和δ-十二碳烯-2-醇的底物的生物氢化，分别生产天然的δ-癸内酯和/或δ-十二烷醇内酯或其混合物，以及 其混合物通过使用选自以下的微生物：德氏酵母酵母（Saccharomyces delbrueckii），欧洲毕赤酵母（Pichia ohmeri），汉逊酵母（Hansenula anomala），毕赤酵母（Desiromyces hansenii），运动发酵单胞菌（Zymomonas mobilis），罗霍氏菌（Zygosaccharomyces rouxii），西葫芦西葫芦（Schawnniomyces occidentalis）以及白纹兰土（Geotrichum candidum））。

公开(公告)号：US5389525A

公开(公告)日：1995-02-14

申请号：US844885

申请日：1992-03-03

申请人： Cornelius P. Hollenberg ,  Zbigniew Janowicz

发明人： Cornelius P. Hollenberg ,  Zbigniew Janowicz

IPC分类号： C12N15/09 ,  C07K14/02 ,  C12N1/19 ,  C12N9/02 ,  C12N9/30 ,  C12N15/81 ,  C12P21/00 ,  C12R1/72 ,  C12R1/78 ,  C12R1/84 ,  C12P21/02 ,  C12N15/52

CPC分类号： C07K14/005 ,  C12N15/815 ,  C12N9/0008 ,  C12N9/242 ,  C12Y102/01002 ,  C12Y102/02001 ,  C12N2730/10122

摘要： The invention relates to DNA-molecules comprising DNA-sequences encoding control regions and the structural gene for a protein having formate dehydrogenase (FMDH) activity. Said DNA-molecules may be combined with DNA-sequences encoding foreign genes so as to bring these genes under the stringent control of the regulation of the FMDH regulatory sequences and/or may be combined to DNA-sequences coding for secretory signals. The invention further relates to recombinant vectors containing said DNA-molecules and micro-organisms containing said vectors or DNA-molecules. Furthermore, the invention relates to a process for producing a useful substance by producing this substance by culturing said micro-organisms and recovering the substance.

摘要翻译： 本发明涉及包含编码对照区的DNA序列的DNA分子和具有甲酸脱氢酶（FMDH）活性的蛋白质的结构基因。 所述DNA分子可以与编码外源基因的DNA序列结合，以使这些基因在调控FMDH调控序列的严格控制下和/或可以与编码分泌信号的DNA序列组合。 本发明还涉及含有所述DNA分子和含有所述载体或DNA分子的微生物的重组载体。 此外，本发明涉及通过培养所述微生物并回收该物质来生产该物质来生产有用物质的方法。

公开(公告)号：US5204248A

公开(公告)日：1993-04-20

申请号：US673553

申请日：1991-03-22

申请人： Takeji Shibatani ,  Takuo Nishida ,  Hiroaki Matsumae

发明人： Takeji Shibatani ,  Takuo Nishida ,  Hiroaki Matsumae

IPC分类号： A61K31/215 ,  A61P9/08 ,  C07C67/31 ,  C07D303/48 ,  C12P7/42 ,  C12P7/62 ,  C12P41/00 ,  C12R1/01 ,  C12R1/06 ,  C12R1/145 ,  C12R1/32 ,  C12R1/465 ,  C12R1/645 ,  C12R1/65 ,  C12R1/72 ,  C12R1/78 ,  C12R1/785 ,  C12R1/85 ,  C12R1/885

CPC分类号： C12P41/00 ,  C07C67/31 ,  C12P7/42 ,  C12P7/62

摘要： Disclosed is a process for preparing 2-halogeno-3-hydroxy-3-phenylpropionic acid ester compounds represented by the formula (I): ##STR1## wherein Ring A is a phenyl group which may be substituted, R.sup.1 is an ester residue, and X is a halogen atom, which comprises permitting an enzyme having the ability of stereoselectively reducing oxo group to hydroxy group to act on 2-halogeno-3-oxo-3-phenylpropionic acid ester compounds represented by the formula (II): ##STR2## wherein Ring A, R.sup.1 and X have the same meanings as defined above.

公开(公告)号：US5008188A

公开(公告)日：1991-04-16

申请号：US117439

申请日：1987-11-04

申请人： Hideaki Yamada ,  Yoshiki Tani ,  Sakayu Shimizu ,  Shozo Shiozaki

发明人： Hideaki Yamada ,  Yoshiki Tani ,  Sakayu Shimizu ,  Shozo Shiozaki

IPC分类号： C12P19/40 ,  C12R1/01 ,  C12R1/05 ,  C12R1/06 ,  C12R1/065 ,  C12R1/13 ,  C12R1/15 ,  C12R1/20 ,  C12R1/265 ,  C12R1/29 ,  C12R1/32 ,  C12R1/365 ,  C12R1/385 ,  C12R1/465 ,  C12R1/62 ,  C12R1/625 ,  C12R1/64 ,  C12R1/645 ,  C12R1/65 ,  C12R1/66 ,  C12R1/77 ,  C12R1/78 ,  C12R1/785 ,  C12R1/80 ,  C12R1/84 ,  C12R1/845 ,  C12R1/88 ,  C12P13/12 ,  C12P19/18

CPC分类号： C12P19/40

摘要： Process for producing S-adenosyl-L-homocysteine, which comprises reacting adenosine with homocysteine by contacting them in an aqueous medium in the presence of cells or treated cells of a microorganism of a specified genus having the ability to synthesize S-adenosyl-L-homocysteine from adenosine and homocysteine, and collecting the S-adenosyl-L-homocysteine synthesized.

摘要翻译： 该方法包括使腺苷与同型半胱氨酸反应，通过在具有合成S-腺苷-L-同型半胱氨酸的能力的特定属的微生物的细胞或处理细胞的存在下在水性介质中接触， 来自腺苷和高半胱氨酸的同型半胱氨酸，并收集合成的S-腺苷-L-高半胱氨酸。

公开(公告)号：US4855231A

公开(公告)日：1989-08-08

申请号：US780102

申请日：1985-09-25

申请人： David W. Stroman ,  Paul F. Brust ,  Steven B. Ellis ,  Thomas R. Gingeras ,  Michael M. Harpold ,  Juerg F. Tschopp

发明人： David W. Stroman ,  Paul F. Brust ,  Steven B. Ellis ,  Thomas R. Gingeras ,  Michael M. Harpold ,  Juerg F. Tschopp

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/00 ,  C07K14/39 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N9/04 ,  C12N15/00 ,  C12N15/15 ,  C12N15/52 ,  C12N15/53 ,  C12N15/54 ,  C12N15/68 ,  C12N15/70 ,  C12N15/81 ,  C12P21/02 ,  C12R1/19 ,  C12R1/645 ,  C12R1/72 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85 ,  C12R1/865 ,  C12R1/88

CPC分类号： C12N9/0006 ,  C12N15/52 ,  C12N15/68 ,  C12N15/70 ,  C12N15/815

摘要： Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention are detailed. The production of polypeptide product under the control of the regulatory regions of the invention is demonstrated.

公开(公告)号：US4247635A

公开(公告)日：1981-01-27

申请号：US100571

申请日：1979-12-05

申请人： Klaus Kieslich ,  Bernd Raduchel ,  Werner Skubalia ,  Helmut Vorbruggen ,  Helmut Dahl

发明人： Klaus Kieslich ,  Bernd Raduchel ,  Werner Skubalia ,  Helmut Vorbruggen ,  Helmut Dahl

IPC分类号： C07D307/935 ,  C12P17/04 ,  C12P31/00 ,  C12R1/645 ,  C12R1/78

CPC分类号： C07D307/935 ,  C12P17/04 ,  C12P31/00 ,  C12R1/645 ,  C12R1/78 ,  C12R1/85 ,  C12R1/86 ,  Y10S435/911 ,  Y10S435/93 ,  Y10S435/941

摘要： A process for the preparation of a 15 .alpha.-hydroxyprostaglandin intermediate of the formula ##STR1## wherein R.sub.1 is phenoxymethyl, phenoxymethyl substituted on the phenyl moiety by halogen or trifluoromethyl, or alkyl of 1-5 carbon atoms, andR.sub.2 is hydrogen, acetyl, benzoyl or p-phenylbenzoyl,which comprises stereospecifically microbiologically reducing a corresponding 15-ketone of the formula ##STR2## with a strain of the microorganism Kloeckera, Saccharomyces or Hansenula.

摘要翻译： 制备式Ia的15α-羟基前列地格兰中间体的方法，其中R1是苯氧基甲基，在苯基部分被卤素或三氟甲基取代的苯氧基甲基或1-5个碳原子的烷基，R 2是氢， 乙酰基，苯甲酰基或对苯基苯甲酰基，其包括用微生物Kloeckera，Saccharomyces或Hansenula的菌株立体专一地微生物还原相应的式II的酮。

公开(公告)号：US3620918A

公开(公告)日：1971-11-16

申请号：US3620918D

申请日：1969-11-21

申请人： TAKASAGO PERFUMERY CO LTD

发明人： MOROE TATSUO ,  HATTORI SATOHIKO ,  KOMATSU AKIRA ,  YAMAGUCHI YUZO

IPC分类号： C12P7/22 ,  A47B95/00 ,  C07C35/12 ,  C12P41/00 ,  C12R1/645 ,  C12R1/72 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85 ,  C12R1/88 ,  C12B1/00

CPC分类号： A47B95/00 ,  C07C35/12 ,  Y10S435/911 ,  Y10S435/921 ,  Y10S435/93 ,  Y10S435/938 ,  Y10S435/94 ,  Y10S435/944

摘要： Method for the biochemical isolation of 1-menthol. Specifically, an improved method for the optical resolution of racemic menthol utilizing an enzyme, carboxylic ester hydrolase, which is produced by micro-organism belonging to the class of Candida, Saccharomyces, Hansenula, Rhodotorula, Torulopsis, Schizosaccharomyces, Pichia, Sporobolomyces, Debaryomyces, Nadsonia, Trichosporon, Brettanomyces, and Cryptococcus. The 1-menthol may be utilized in perfumes, medicines, etc.

公开(公告)号：US12091658B2

公开(公告)日：2024-09-17

申请号：US17276186

申请日：2019-09-23

申请人： ORTA DOGU TEKNIK UNIVERSITESI

发明人： Pinar Calik ,  Burcu Gunduz Ergun

IPC分类号： C12N15/10 ,  C07K14/39 ,  C12N1/14 ,  C12N15/81 ,  C12R1/72 ,  C12R1/78 ,  C12R1/84 ,  C12R1/88

CPC分类号： C12N15/102 ,  C07K14/39 ,  C12N1/14 ,  C12N15/815 ,  C12R2001/72 ,  C12R2001/78 ,  C12R2001/84 ,  C12R2001/88

摘要： Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter variants include at least one of the specified modifications on wild-type Pichia pastoris ADH2 promoter (SEQ ID NO: 1). The modification includes one of the following mutations: integration of a Cat8 transcription factor binding site (TFBS), particularly integration of SEQ ID NO: 3 or other gene sequences that show at least 80% similarity with this sequence, at any positions within nucleotides a) 647 to 660; b) 739 to 752; c) 1 to 948; and d) mutations specified with SEQ ID NO: 2 within nucleotides 15 to 848 separately and combinations thereof.

公开(公告)号：US20240239854A1

公开(公告)日：2024-07-18

申请号：US18392130

申请日：2023-12-21

申请人： Donald Danforth Plant Science Center

发明人： Kirk Czymmek ,  Dilip M. Shah ,  Siva L.S. Velivelli ,  Saurav Misra ,  Hui Li ,  Meenakshi Tetorya

IPC分类号： C07K14/47 ,  C12N1/16 ,  C12R1/72 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85

CPC分类号： C07K14/4723 ,  C12N1/16 ,  C12R2001/72 ,  C12R2001/78 ,  C12R2001/84 ,  C12R2001/85

摘要： Antimicrobial modified defensin or defensin-like peptides, modified C-terminal fragments of a defensin or defensin-like peptides and nucleic acids encoding the same are disclosed. Compositions comprising the defensin variant peptides and methods of their use to control microbial infections of plants and vertebrate subjects as well as contamination of feedstuffs and foodstuffs are also disclosed.