公开(公告)号：US4740306A

公开(公告)日：1988-04-26

申请号：US840399

申请日：1986-03-17

申请人： Gerald Litwack ,  Ted M. Kirsch

发明人： Gerald Litwack ,  Ted M. Kirsch

IPC分类号： B01J39/26 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  B01D15/08

CPC分类号： B01J39/26 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  Y10S435/803 ,  Y10S435/815

摘要： A chromatographic column and process for isolating or purifying steroid hormone or cell membrane receptors using the column are provided in which the column contains at least three resin layers between the inlet and the outlet ends of the column, the layer closest the inlet being a strong cationic exchange resin, the middle layer being a matrix containing a triazine dye that will bind proteases and proteins with dinucleotide fold conformations, and the layer closest the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.

摘要翻译： 提供色谱柱和使用该柱分离或纯化类固醇激素或细胞膜受体的方法，其中柱在柱的入口端和出口端之间至少含有三个树脂层，最靠近入口的层是强阳离子 交换树脂，中间层是含有三嗪染料的基质，其将与二核苷酸折叠构象结合蛋白酶和蛋白质，最靠近出口的是弱阴离子交换树脂，树脂层的体积比为1:2 ：1。